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    MED20 is essential for early embryogenesis and regulates NANOG expression

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    _17417899___Reproduction__MED2 ...
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    Authors
    Cui, Wei
    Marcho, Chelsea
    Wang, Yongsheng
    Degani, Rinat
    Golan, Morgane
    Tremblay, Kimberly D.
    Rivera-Pérez, Jaime A.
    Mager, Jesse
    UMass Chan Affiliations
    Rivera Lab
    Division of Genes and Development, Department of Pediatrics
    Document Type
    Journal Article
    Publication Date
    2019-03-01
    Keywords
    Mediator Med20
    Mouse embryo
    Nanog
    Trophectoderm
    Cell lineage specification
    Cell Biology
    Developmental Biology
    Nucleic Acids, Nucleotides, and Nucleosides
    Physiological Processes
    Reproductive and Urinary Physiology
    
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    Link to Full Text
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6545164/
    Abstract
    Mediator is an evolutionarily conserved multi-subunit complex, bridging transcriptional activators and repressors to the general RNA polymerase II (Pol II) initiation machinery. Though the Mediator complex is crucial for the transcription of almost all Pol II promoters in eukaryotic organisms, the phenotypes of individual Mediator subunit mutants are each distinct. Here, we report for the first time, the essential role of subunit MED20 in early mammalian embryo development. Although Med20 mutant mouse embryos exhibit normal morphology at E3.5 blastocyst stage, they cannot be recovered at early post-gastrulation stages. Outgrowth assays show that mutant blastocysts cannot hatch from the zona pellucida, indicating impaired blastocyst function. Assessments of cell death and cell lineage specification reveal that apoptosis, inner cell mass, trophectoderm and primitive endoderm markers are normal in mutant blastocysts. However, the epiblast marker NANOG is ectopically expressed in the trophectoderm of Med20 mutants, indicative of defects in trophoblast specification. These results suggest that MED20 specifically, and the Mediator complex in general, are essential for the earliest steps of mammalian development and cell lineage specification.
    Source

    Cui W, Marcho C, Wang Y, Degani R, Golan M, Tremblay KD, Rivera-Pérez JA, Mager J. MED20 is essential for early embryogenesis and regulates NANOG expression. Reproduction. 2019 Mar;157(3):215-222. doi: 10.1530/REP-18-0508. PMID: 30571656; PMCID: PMC6545164. Link to article on publisher's site

    DOI
    10.1530/REP-18-0508
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/29503
    PubMed ID
    30571656
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    Link to Article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1530/REP-18-0508
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