The RNA phosphatase PIR-1 regulates endogenous small RNA pathways in C. elegans [preprint]
AuthorsChaves, Daniel A.
Moresco, James J.
Eun Oh, Myung
Conte, Darryl Jr.
Yates, John R. III
Mello, Craig C.
RNA phosphatase PIR-1
endogenous small RNA pathways
Cell and Developmental Biology
Enzymes and Coenzymes
Nucleic Acids, Nucleotides, and Nucleosides
MetadataShow full item record
AbstractEukaryotic cells regulate 5' triphosphorylated (ppp-) RNAs to promote cellular functions and prevent recognition by antiviral RNA sensors. For example, RNA capping enzymes possess triphosphatase domains that remove the γ phosphates of ppp-RNAs during RNA capping. Members of the closely related PIR1 family of RNA polyphosphatases remove both the β and γ phosphates from ppp-RNAs. Here we show that C. elegans PIR-1 dephosphorylates ppp-RNAs made by cellular RdRPs and is required for the maturation of 26G-RNAs, Dicer-dependent small RNAs that regulate thousands of genes during spermatogenesis and embryogenesis. PIR-1 also regulates the CSR-1 22G-RNA pathway and has critical functions in both somatic and germline development. Our findings suggest that PIR-1 modulates both Dicer-dependent and -independent Argonaute pathways, and provide insight into how cells and viruses use a conserved RNA phosphatase to regulate and respond to ppp-RNA species.
bioRxiv 2020.08.03.235143; doi: https://doi.org/10.1101/2020.08.03.235143. Link to preprint on bioRxiv service.
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/29513
RightsThe copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.
Except where otherwise noted, this item's license is described as The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.