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dc.contributor.authorFazzalari, Amanda
dc.contributor.authorBasadonna, Giacomo
dc.contributor.authorKucukural, Alper
dc.contributor.authorTanriverdi, Kahraman
dc.contributor.authorKoupenova-Zamor, Milka
dc.contributor.authorDickson, Eric W.
dc.contributor.authorBlackwood, Meghan
dc.contributor.authorMueller, Christian
dc.contributor.authorFreedman, Jane E.
dc.contributor.authorCahan, Mitchell A.
dc.date2022-08-11T08:08:25.000
dc.date.accessioned2022-08-23T15:54:33Z
dc.date.available2022-08-23T15:54:33Z
dc.date.issued2020-08-25
dc.date.submitted2020-09-17
dc.identifier.citation<p>Fazzalari A, Basadonna G, Kucukural A, Tanriverdi K, Koupenova M, Pozzi N, Kakuturu J, Friedrich AU, Korstanje R, Fowler N, Belant JL, Beyer DE Jr, Brooks MB, Dickson EW, Blackwood M, Mueller C, Palesty JA, Freedman JE, Cahan MA. A Translational Model for Venous Thromboembolism: MicroRNA Expression in Hibernating Black Bears. J Surg Res. 2020 Aug 25;257:203-212. doi: 10.1016/j.jss.2020.06.027. Epub ahead of print. PMID: 32858321. <a href="https://doi.org/10.1016/j.jss.2020.06.027">Link to article on publisher's site</a></p>
dc.identifier.issn0022-4804 (Linking)
dc.identifier.doi10.1016/j.jss.2020.06.027
dc.identifier.pmid32858321
dc.identifier.urihttp://hdl.handle.net/20.500.14038/29578
dc.description<p>Full author list omitted for brevity. For the full list of authors, see article.</p>
dc.description.abstractBACKGROUND: Hibernating American black bears have significantly different clotting parameters than their summer active counterparts, affording them protection against venous thromboembolism during prolonged periods of immobility. We sought to evaluate if significant differences exist between the expression of microRNAs in the plasma of hibernating black bears compared with their summer active counterparts, potentially contributing to differences in hemostasis during hibernation. MATERIALS AND METHODS: MicroRNA sequencing was assessed in plasma from 21 American black bears in summer active (n = 11) and hibernating states (n = 10), and microRNA signatures during hibernating and active state were established using both bear and human genome. MicroRNA targets were predicted using messenger RNA (mRNA) transcripts from black bear kidney cells. In vitro studies were performed to confirm the relationship between identified microRNAs and mRNA expression, using artificial microRNA and human liver cells. RESULTS: Using the bear genome, we identified 15 microRNAs differentially expressed in the plasma of hibernating black bears. Of these microRNAs, three were significantly downregulated (miR-141-3p, miR-200a-3p, and miR-200c-3p), were predicted to target SERPINC1, the gene for antithrombin, and demonstrated regulatory control of the gene mRNA expression in cell studies. CONCLUSIONS: Our findings suggest that the hibernating black bears' ability to maintain hemostasis and achieve protection from venous thromboembolism during prolonged periods of immobility may be due to changes in microRNA signatures and possible upregulation of antithrombin expression.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=32858321&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1016/j.jss.2020.06.027
dc.subjectAnticoagulation
dc.subjectAntithrombin
dc.subjectThrombosis
dc.subjectVenous thromboembolism
dc.subjectmicroRNA
dc.subjectBiological Phenomena, Cell Phenomena, and Immunity
dc.subjectCardiovascular Diseases
dc.subjectCirculatory and Respiratory Physiology
dc.subjectDisease Modeling
dc.subjectGenetics and Genomics
dc.subjectMolecular Biology
dc.subjectNucleic Acids, Nucleotides, and Nucleosides
dc.subjectPhysiological Processes
dc.subjectSurgery
dc.subjectTranslational Medical Research
dc.titleA Translational Model for Venous Thromboembolism: MicroRNA Expression in Hibernating Black Bears
dc.typeJournal Article
dc.source.journaltitleThe Journal of surgical research
dc.source.volume257
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/faculty_pubs/1799
dc.identifier.contextkey19432922
html.description.abstract<p>BACKGROUND: Hibernating American black bears have significantly different clotting parameters than their summer active counterparts, affording them protection against venous thromboembolism during prolonged periods of immobility. We sought to evaluate if significant differences exist between the expression of microRNAs in the plasma of hibernating black bears compared with their summer active counterparts, potentially contributing to differences in hemostasis during hibernation.</p> <p>MATERIALS AND METHODS: MicroRNA sequencing was assessed in plasma from 21 American black bears in summer active (n = 11) and hibernating states (n = 10), and microRNA signatures during hibernating and active state were established using both bear and human genome. MicroRNA targets were predicted using messenger RNA (mRNA) transcripts from black bear kidney cells. In vitro studies were performed to confirm the relationship between identified microRNAs and mRNA expression, using artificial microRNA and human liver cells.</p> <p>RESULTS: Using the bear genome, we identified 15 microRNAs differentially expressed in the plasma of hibernating black bears. Of these microRNAs, three were significantly downregulated (miR-141-3p, miR-200a-3p, and miR-200c-3p), were predicted to target SERPINC1, the gene for antithrombin, and demonstrated regulatory control of the gene mRNA expression in cell studies.</p> <p>CONCLUSIONS: Our findings suggest that the hibernating black bears' ability to maintain hemostasis and achieve protection from venous thromboembolism during prolonged periods of immobility may be due to changes in microRNA signatures and possible upregulation of antithrombin expression.</p>
dc.identifier.submissionpathfaculty_pubs/1799
dc.contributor.departmentDepartment of Pediatrics
dc.contributor.departmentMueller Lab for Gene Therapy, Horae Gene Therapy Center
dc.contributor.departmentDepartment of Emergency Medicine
dc.contributor.departmentDivision of Cardiovascular Medicine, Department of Medicine
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentBioinformatics Core
dc.contributor.departmentDepartment of Surgery
dc.source.pages203-212


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