Implantation and Gastrulation Abnormalities Characterize Early Embryonic Lethal Mouse Lines [preprint]
UMass Chan AffiliationsDepartment of Pediatrics, Division of Genes and Development
Female Urogenital Diseases and Pregnancy Complications
Genetics and Genomics
Male Urogenital Diseases
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AbstractThe period of development between the zygote and embryonic day 9.5 in mice includes multiple developmental milestones essential for embryogenesis. The preeminence of this period of development has been illustrated in loss of function studies conducted by the International Mouse Phenotyping Consortium (IMPC) which have shown that close to one third of all mouse genes are essential for survival to weaning age and a significant number of mutations cause embryo lethality before E9.5. Here we report a systematic analysis of 21 pre-E9.5 lethal lines generated by the IMPC. Analysis of pre- and post-implantation embryos revealed that the majority of the lines exhibit mutant phenotypes that fall within a window of development between implantation and gastrulation with few pre-implantation and no post-gastrulation phenotypes. Our study provides multiple genetic inroads into the molecular mechanisms that control early mammalian development and the etiology of human disease, in particular, the genetic bases of infertility and pregnancy loss. We propose a strategy for an efficient assessment of early embryonic lethal mutations that can be used to assign phenotypes to developmental milestones and outline the time of lethality.
bioRxiv 2020.10.08.331587; doi: https://doi.org/10.1101/2020.10.08.331587. Link to preprint on bioRxiv
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/29597
This article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.
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Except where otherwise noted, this item's license is described as The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.