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dc.contributor.authorSchulte, Fabian
dc.contributor.authorKing, Oliver D.
dc.contributor.authorPaster, Bruce J.
dc.contributor.authorMoscicki, Anna-Barbara
dc.contributor.authorYao, Tzy-Jyun
dc.contributor.authorVan Dyke, Russell B.
dc.contributor.authorShiboski, Caroline
dc.contributor.authorRyder, Mark
dc.contributor.authorSeage, George
dc.contributor.authorHardt, Markus
dc.date2022-08-11T08:08:25.000
dc.date.accessioned2022-08-23T15:54:44Z
dc.date.available2022-08-23T15:54:44Z
dc.date.issued2020-09-11
dc.date.submitted2020-11-16
dc.identifier.citation<p>Schulte F, King OD, Paster BJ, Moscicki AB, Yao TJ, Van Dyke RB, Shiboski C, Ryder M, Seage G, Hardt M; Pediatric HIV/AIDS Cohort Study. Salivary metabolite levels in perinatally HIV-infected youth with periodontal disease. Metabolomics. 2020 Sep 11;16(9):98. doi: 10.1007/s11306-020-01719-6. PMID: 32915320. <a href="https://doi.org/10.1007/s11306-020-01719-6">Link to article on publisher's site</a></p>
dc.identifier.issn1573-3882 (Linking)
dc.identifier.doi10.1007/s11306-020-01719-6
dc.identifier.pmid32915320
dc.identifier.urihttp://hdl.handle.net/20.500.14038/29613
dc.description.abstractINTRODUCTION: Salivary metabolite profiles are altered in adults with HIV compared to their uninfected counterparts. Less is known about youth with HIV and how oral disorders that commonly accompany HIV infection impact salivary metabolite levels. OBJECTIVE: As part of the Adolescent Master Protocol multi-site cohort study of the Pediatric HIV/AIDS Cohort Study (PHACS) network we compared the salivary metabolome of youth with perinatally-acquired HIV (PHIV) and youth HIV-exposed, but uninfected (PHEU) and determined whether metabolites differ in PHIV versus PHEU. METHODS: We used three complementary targeted and discovery-based liquid chromatography-tandem mass spectrometry (LC-MS/MS) workflows to characterize salivary metabolite levels in 20 PHIV and 20 PHEU youth with and without moderate periodontitis. We examined main effects associated with PHIV and periodontal disease, and the interaction between them. RESULTS: We did not identify differences in salivary metabolite profiles that remained significant under stringent control for both multiple between-group comparisons and multiple metabolites. Levels of cadaverine, a known periodontitis-associated metabolite, were more abundant in individuals with periodontal disease with the difference being more pronounced in PHEU than PHIV. In the discovery-based dataset, we identified a total of 564 endogenous peptides in the metabolite extracts, showing that proteolytic processing and amino acid metabolism are important to consider in the context of HIV infection. CONCLUSION: The salivary metabolite profiles of PHIV and PHEU youth were overall very similar. Individuals with periodontitis particularly among the PHEU youth had higher levels of cadaverine, suggesting that HIV infection, or its treatment, may influence the metabolism of oral bacteria.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=32915320&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1007/s11306-020-01719-6
dc.subjectBiomarkers
dc.subjectHAART
dc.subjectHIV infection
dc.subjectMass spectrometry
dc.subjectPeriodontal disease
dc.subjectTargeted metabolomics
dc.subjectBiochemical Phenomena, Metabolism, and Nutrition
dc.subjectBiological Factors
dc.subjectMaternal and Child Health
dc.subjectPediatrics
dc.subjectStomatognathic Diseases
dc.subjectVirus Diseases
dc.titleSalivary metabolite levels in perinatally HIV-infected youth with periodontal disease
dc.typeArticle
dc.source.journaltitleMetabolomics : Official journal of the Metabolomic Society
dc.source.volume16
dc.source.issue9
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/faculty_pubs/1830
dc.identifier.contextkey20206049
html.description.abstract<p>INTRODUCTION: Salivary metabolite profiles are altered in adults with HIV compared to their uninfected counterparts. Less is known about youth with HIV and how oral disorders that commonly accompany HIV infection impact salivary metabolite levels.</p> <p>OBJECTIVE: As part of the Adolescent Master Protocol multi-site cohort study of the Pediatric HIV/AIDS Cohort Study (PHACS) network we compared the salivary metabolome of youth with perinatally-acquired HIV (PHIV) and youth HIV-exposed, but uninfected (PHEU) and determined whether metabolites differ in PHIV versus PHEU.</p> <p>METHODS: We used three complementary targeted and discovery-based liquid chromatography-tandem mass spectrometry (LC-MS/MS) workflows to characterize salivary metabolite levels in 20 PHIV and 20 PHEU youth with and without moderate periodontitis. We examined main effects associated with PHIV and periodontal disease, and the interaction between them.</p> <p>RESULTS: We did not identify differences in salivary metabolite profiles that remained significant under stringent control for both multiple between-group comparisons and multiple metabolites. Levels of cadaverine, a known periodontitis-associated metabolite, were more abundant in individuals with periodontal disease with the difference being more pronounced in PHEU than PHIV. In the discovery-based dataset, we identified a total of 564 endogenous peptides in the metabolite extracts, showing that proteolytic processing and amino acid metabolism are important to consider in the context of HIV infection.</p> <p>CONCLUSION: The salivary metabolite profiles of PHIV and PHEU youth were overall very similar. Individuals with periodontitis particularly among the PHEU youth had higher levels of cadaverine, suggesting that HIV infection, or its treatment, may influence the metabolism of oral bacteria.</p>
dc.identifier.submissionpathfaculty_pubs/1830
dc.contributor.departmentWellstone Center for FSHD
dc.contributor.departmentDepartment of Neurology
dc.source.pages98


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