RNA-seq with RNase H-based ribosomal RNA depletion specifically designed for C. elegans
Abstract
Here we describe a rRNA depletion protocol based on RNase H digestion using antisense oligonucleotides (ASOs) specifically designed for C. elegans cytoplasmic rRNA (Fig. 1A). We suggest that this rRNA depletion protocol is applicable to RNA-seq applications where the yield of mRNA enrichment should be independent of poly(A) status, or any application which benefits from the removal of rRNA sequences, such ribosome profiling, or sequencing of non-coding RNAs other than rRNA.Source
Duan Y, Sun Y, Ambros V. RNA-seq with RNase H-based ribosomal RNA depletion specifically designed for C. elegans. MicroPubl Biol. 2020 Sep 22;2020:10.17912/micropub.biology.000312. doi: 10.17912/micropub.biology.000312. PMID: 33005886; PMCID: PMC7520128. Link to article on publisher's site
DOI
10.17912/micropub.biology.000312Permanent Link to this Item
http://hdl.handle.net/20.500.14038/29690PubMed ID
33005886Related Resources
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Copyright: © 2020 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Distribution License
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Except where otherwise noted, this item's license is described as Copyright: © 2020 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.