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Gene transfer in skeletal and cardiac muscle using recombinant adeno-associated virus

dc.contributor.authorGruntman, Alisha M.
dc.contributor.authorBish, Lawrence T.
dc.contributor.authorMueller, Christian
dc.contributor.authorSweeney, H. Lee
dc.contributor.authorFlotte, Terence R.
dc.contributor.authorGao, Guangping
dc.date2022-08-11T08:08:26.000
dc.date.accessioned2022-08-23T15:55:11Z
dc.date.available2022-08-23T15:55:11Z
dc.date.issued2013-02-01
dc.date.submitted2013-07-09
dc.identifier.citation<p>Curr Protoc Microbiol. 2013 Feb;Chapter 14:Unit 14D.3. doi: 10.1002/9780471729259.mc14d03s28.<a href="http://dx.doi.org/10.1002/9780471729259.mc14d03s28" target="_blank"> Link to article on publisher's site</a></p>
dc.identifier.issn1934-8533 (Electronic)
dc.identifier.doi10.1002/9780471729259.mc14d03s28
dc.identifier.pmid23408131
dc.identifier.urihttp://hdl.handle.net/20.500.14038/29697
dc.description.abstractAdeno-associated virus (AAV) is a DNA virus with a small ( approximately 4.7 kb) single-stranded genome. It is a naturally replication-defective parvovirus of the dependovirus group. Recombinant AAV (rAAV), for use as a gene transfer vector, is created by replacing the viral rep and cap genes with the transgene of interest along with promoter and polyadenylation sequences. Only the viral inverted terminal repeats (ITRs) are required in cis for replication and packaging during production. The ITRs are also necessary and sufficient for vector genome processing and persistence during transduction. The tissue tropism of the rAAV vector is determined by the AAV capsid. In this unit we will discuss several methods to deliver rAAV in order to transduce cardiac and/or skeletal muscle, including intravenous delivery, intramuscular delivery, isolated limb infusion, intrapericardial injection in neonatal mice, and left ventricular wall injection in adult rats.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=23408131&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3641885/
dc.subjectDependovirus
dc.subjectGenetic Vectors
dc.subjectGenetic Therapy
dc.subjectGene Transfer Techniques
dc.subjectMuscle, Skeletal
dc.subjectMyocardium
dc.subjectUMCCTS funding
dc.subjectGenetics and Genomics
dc.subjectMicrobiology
dc.subjectMolecular Genetics
dc.titleGene transfer in skeletal and cardiac muscle using recombinant adeno-associated virus
dc.typeBook Chapter
dc.source.booktitleCurrent protocols in microbiology
dc.source.volumeChapter 14
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/faculty_pubs/191
dc.identifier.contextkey4297403
html.description.abstract<p>Adeno-associated virus (AAV) is a DNA virus with a small ( approximately 4.7 kb) single-stranded genome. It is a naturally replication-defective parvovirus of the dependovirus group. Recombinant AAV (rAAV), for use as a gene transfer vector, is created by replacing the viral rep and cap genes with the transgene of interest along with promoter and polyadenylation sequences. Only the viral inverted terminal repeats (ITRs) are required in cis for replication and packaging during production. The ITRs are also necessary and sufficient for vector genome processing and persistence during transduction. The tissue tropism of the rAAV vector is determined by the AAV capsid. In this unit we will discuss several methods to deliver rAAV in order to transduce cardiac and/or skeletal muscle, including intravenous delivery, intramuscular delivery, isolated limb infusion, intrapericardial injection in neonatal mice, and left ventricular wall injection in adult rats.</p>
dc.identifier.submissionpathfaculty_pubs/191
dc.contributor.departmentDepartment of Pediatrics
dc.contributor.departmentDepartment of Microbiology and Physiological Systems
dc.contributor.departmentGene Therapy Center
dc.source.pagesUnit 14D.3


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