A genome wide RNA interference screening method to identify host factors that modulate influenza A virus replication
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UMass Chan Affiliations
Department of Microbiology and Physiological SystemsDocument Type
Journal ArticlePublication Date
2013-02-01Keywords
siRNA screeningAutomated siRNA transfection
Reverse transfection
Functional genomics
Host-virus interactions
Influenza A virus
Whole genome screen
Genetics and Genomics
Genomics
Molecular BiologyMolecular GeneticsNucleic Acids, Nucleotides, and NucleosidesVirologyViruses
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The use of genome wide RNA interference (RNAi) screens to investigate host-virals interactions has revealed unexpected connections that have improved our understanding of viral pathogenesis and cell biology. This work describes the use of an RNAi screening method employing an immunofluorescence image-based strategy and influenza A virus. We find this approach to be readily implemented, scalable and amenable to the direct evaluation of a variety of viral lifecycles.Source
Methods. 2013 Feb;59(2):217-24. doi: 10.1016/j.ymeth.2012.09.009. Link to article on publisher's site
DOI
10.1016/j.ymeth.2012.09.009Permanent Link to this Item
http://hdl.handle.net/20.500.14038/29769PubMed ID
23036328Related Resources
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