A genome wide RNA interference screening method to identify host factors that modulate influenza A virus replication
UMass Chan AffiliationsDepartment of Microbiology and Physiological Systems
Document TypeJournal Article
Automated siRNA transfection
Influenza A virus
Whole genome screen
Genetics and Genomics
Nucleic Acids, Nucleotides, and Nucleosides
MetadataShow full item record
AbstractThe use of genome wide RNA interference (RNAi) screens to investigate host-virals interactions has revealed unexpected connections that have improved our understanding of viral pathogenesis and cell biology. This work describes the use of an RNAi screening method employing an immunofluorescence image-based strategy and influenza A virus. We find this approach to be readily implemented, scalable and amenable to the direct evaluation of a variety of viral lifecycles.
Methods. 2013 Feb;59(2):217-24. doi: 10.1016/j.ymeth.2012.09.009. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/29769
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