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dc.contributor.authorChin, Christopher R.
dc.contributor.authorBrass, Abraham L.
dc.date2022-08-11T08:08:26.000
dc.date.accessioned2022-08-23T15:55:30Z
dc.date.available2022-08-23T15:55:30Z
dc.date.issued2013-02-01
dc.date.submitted2013-07-25
dc.identifier.citation<p>Methods. 2013 Feb;59(2):217-24. doi: 10.1016/j.ymeth.2012.09.009. <a href="http://dx.doi.org/10.1016/j.ymeth.2012.09.009">Link to article on publisher's site</a></p>
dc.identifier.issn1046-2023 (Linking)
dc.identifier.doi10.1016/j.ymeth.2012.09.009
dc.identifier.pmid23036328
dc.identifier.urihttp://hdl.handle.net/20.500.14038/29769
dc.description.abstractThe use of genome wide RNA interference (RNAi) screens to investigate host-virals interactions has revealed unexpected connections that have improved our understanding of viral pathogenesis and cell biology. This work describes the use of an RNAi screening method employing an immunofluorescence image-based strategy and influenza A virus. We find this approach to be readily implemented, scalable and amenable to the direct evaluation of a variety of viral lifecycles.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=23036328&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttp://dx.doi.org/10.1016/j.ymeth.2012.09.009
dc.subjectsiRNA screening
dc.subjectAutomated siRNA transfection
dc.subjectReverse transfection
dc.subjectFunctional genomics
dc.subjectHost-virus interactions
dc.subjectInfluenza A virus
dc.subjectWhole genome screen
dc.subjectGenetics and Genomics
dc.subjectGenomics
dc.subjectMolecular Biology
dc.subjectMolecular Genetics
dc.subjectNucleic Acids, Nucleotides, and Nucleosides
dc.subjectVirology
dc.subjectViruses
dc.titleA genome wide RNA interference screening method to identify host factors that modulate influenza A virus replication
dc.typeJournal Article
dc.source.journaltitleMethods (San Diego, Calif.)
dc.source.volume59
dc.source.issue2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/faculty_pubs/198
dc.identifier.contextkey4349475
html.description.abstract<p>The use of genome wide RNA interference (RNAi) screens to investigate host-virals interactions has revealed unexpected connections that have improved our understanding of viral pathogenesis and cell biology. This work describes the use of an RNAi screening method employing an immunofluorescence image-based strategy and influenza A virus. We find this approach to be readily implemented, scalable and amenable to the direct evaluation of a variety of viral lifecycles.</p>
dc.identifier.submissionpathfaculty_pubs/198
dc.contributor.departmentDepartment of Microbiology and Physiological Systems
dc.source.pages217-24


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