The N-terminus of vaccinia virus host range protein C7L is essential for function
UMass Chan Affiliations
Department of Medicine, Division of Infectious Diseases and ImmunologyDepartment of Pathology
Document Type
Journal ArticlePublication Date
2013-02-01Keywords
DNA Mutational Analysis*Host Specificity
Recombination, Genetic
Vaccinia virus
Viral Proteins
Vaccinia virus
Host range gene
C7L
Poxvirus
Immunomodulatory gene
Immunology and Infectious Disease
Immunoprophylaxis and Therapy
Medical Microbiology
Molecular Genetics
Virology
Metadata
Show full item recordAbstract
Vaccinia virus (VACV), a member of the Poxviridae family of large double-stranded DNA viruses, is being used as a smallpox vaccine as well as an expression vector for immunization against other infectious diseases and cancer. The host range of wild type VACV is very broad among mammalian cells. C7L is a host range gene identified in VACV and is well conserved in mammalian poxviruses except for parapoxviruses and molluscum contagiosum virus. The molecular mechanisms by which the C7L gene exerts host range function are not well understood. The C7L protein does not have any known conserved domains or show sequence similarity to cellular proteins or viral proteins other than the C7L homologs in mammalian poxviruses. We generated recombinant vaccinia viruses carrying deletion mutants of the C7L gene using NYVAC as a parental strain and found that the N-terminus is essential for host range function of C7L, which is consistent with a previous report that showed that homology among C7L homologs are greater near the N-terminus than the C-terminus.Source
Virus Genes. 2013 Feb;46(1):20-7. doi: 10.1007/s11262-012-0822-x. Link to article on publisher's siteDOI
10.1007/s11262-012-0822-xPermanent Link to this Item
http://hdl.handle.net/20.500.14038/29800PubMed ID
23001690Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1007/s11262-012-0822-x