Pseudomonas aeruginosa PA14 produces R-bodies, extendable protein polymers with roles in host colonization and virulence [preprint]
Authors
Wang, BryanLin, Yu-Cheng
Vasquez-Rifo, Alejandro
Jo, Jeanyoung
Price-Whelan, Alexa
Tao McDonald, Shujuan
Brown, Lewis M.
Sieben, Christian
Dietrich, Lars E.P.
UMass Chan Affiliations
Program in Molecular MedicineDocument Type
PreprintPublication Date
2021-04-29Keywords
MicrobiologyPseudomonas aeruginosa
protein polymers
R-bodies
Amino Acids, Peptides, and Proteins
Bacteria
Bacterial Infections and Mycoses
Bacteriology
Pathogenic Microbiology
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Show full item recordAbstract
Pseudomonas aeruginosa PA14, an opportunistic pathogen of diverse hosts, contains genes with the potential to confer production of R-bodies (i.e., a “reb cluster”). R-bodies are large, extendable protein polymers best known for their role in killing of paramecia by the bacterium Caedibacter taeniospiralis, and genes in the reb cluster have been implicated in PA14 virulence. Here, we present evidence that PA14 expresses reb cluster genes during colonization of plant and nematode hosts. We identify products of the reb cluster that are R-body-associated and that control stochastic expression of R-body structural genes. We also show that R-body production is required for full virulence in nematodes. Analyses of nematode ribosome content and immune response indicate that R-bodies act via a mechanism involving ribosome cleavage and translational inhibition. These observations provide insight into the biology of R-body production and its consequences during P. aeruginosa infection.Source
bioRxiv 2020.10.26.356394; doi: https://doi.org/10.1101/2020.10.26.356394. Link to preprint on bioRxiv.
DOI
10.1101/2020.10.26.356394Permanent Link to this Item
http://hdl.handle.net/20.500.14038/29810Notes
This article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.
The PDF available for download is Version 3 of this preprint. The complete version history of this preprint is available at bioRxiv.
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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.Distribution License
http://creativecommons.org/licenses/by-nc-nd/4.0/ae974a485f413a2113503eed53cd6c53
10.1101/2020.10.26.356394
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Except where otherwise noted, this item's license is described as The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.