Show simple item record

dc.contributor.authorSmith, Ciearra B.
dc.contributor.authorvan der Vinne, Vincent
dc.contributor.authorMcCartney, Eleanor
dc.contributor.authorStowie, Adam C.
dc.contributor.authorLeise, Tanya L.
dc.contributor.authorMartin-Burgos, Blanca
dc.contributor.authorMolyneux, Penny C. C.
dc.contributor.authorGarbutt, Lauren A.
dc.contributor.authorBrodsky, Michael H.
dc.contributor.authorDavidson, Alec J.
dc.contributor.authorHarrington, Mary E.
dc.contributor.authorDallmann, Robert
dc.contributor.authorWeaver, David R.
dc.date2022-08-11T08:08:27.000
dc.date.accessioned2022-08-23T15:55:42Z
dc.date.available2022-08-23T15:55:42Z
dc.date.issued2021-04-05
dc.date.submitted2021-06-28
dc.identifier.citationCell-type specific circadian bioluminescence rhythms in Dbp reporter mice. Ciearra B. Smith, Vincent van der Vinne, Eleanor McCartney, Adam C. Stowie, Tanya L. Leise, Blanca Martin-Burgos, Penny C. Molyneux, Lauren A. Garbutt, Michael H. Brodsky, Alec J. Davidson, Mary E. Harrington, Robert Dallmann, David R. Weaver bioRxiv 2021.04.04.438413; doi: https://doi.org/10.1101/2021.04.04.438413
dc.identifier.doi10.1101/2021.04.04.438413
dc.identifier.urihttp://hdl.handle.net/20.500.14038/29815
dc.description<p>This article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.</p> <p>The PDF available for download is Version 1 of this preprint. The complete version history of this preprint is available at https://doi.org/10.1101/2021.04.04.438413.</p>
dc.description.abstractCircadian rhythms are endogenously generated physiological and molecular rhythms with a cycle length of about 24 h. Bioluminescent reporters have been exceptionally useful for studying circadian rhythms in numerous species. Here, we report development of a reporter mouse generated by modification of a widely expressed and highly rhythmic gene encoding D-site albumin promoter binding protein (Dbp). In this line of mice, firefly luciferase is expressed from the Dbp locus in a Cre-recombinase- dependent manner, allowing assessment of bioluminescence rhythms in specific cellular populations. A mouse line in which luciferase expression was Cre-independent was also generated. The Dbp reporter alleles do not alter Dbp gene expression rhythms in liver or circadian locomotor activity rhythms. In vitro and in vivo studies show the utility of the reporter alleles for monitoring rhythmicity. Our studies reveal cell-type specific characteristics of rhythms among neuronal populations within the suprachiasmatic nuclei in vitro. In vivo studies show stable Dbp-driven bioluminescence rhythms in the liver of Albumin-Cre;DbpKI/+ “liver reporter” mice. After a shift of the lighting schedule, locomotor activity achieved the proper phase relationship with the new lighting cycle more rapidly than hepatic bioluminescence did. As previously shown, restricting food access to the daytime altered the phase of hepatic rhythmicity. Our model allowed assessment of the rate of recovery from misalignment once animals were provided with food ad libitum. These studies provide clear evidence for circadian misalignment following environmental perturbations and reveal the utility of this model for minimally invasive, longitudinal monitoring of rhythmicity from specific mouse tissues.
dc.language.isoen_US
dc.relation<p>Now published in Journal of Biological Rhythms, doi: https://doi.org/10.1177/07487304211069452.</p>
dc.relation.urlhttps://doi.org/10.1101/2021.04.04.438413
dc.rightsThe copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectPhysiology
dc.subjectCircadian rhythms
dc.subjectCellular and Molecular Physiology
dc.subjectNeuroscience and Neurobiology
dc.titleCell-type specific circadian bioluminescence rhythms recorded from Dbp reporter mice reveal circadian oscillator misalignment [preprint]
dc.typePreprint
dc.source.journaltitlebioRxiv
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=3042&amp;context=faculty_pubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/faculty_pubs/2023
dc.identifier.contextkey23563680
refterms.dateFOA2022-08-23T15:55:42Z
html.description.abstract<p><p id="x-x-x-x-x-p-18">Circadian rhythms are endogenously generated physiological and molecular rhythms with a cycle length of about 24 h. Bioluminescent reporters have been exceptionally useful for studying circadian rhythms in numerous species. Here, we report development of a reporter mouse generated by modification of a widely expressed and highly rhythmic gene encoding D-site albumin promoter binding protein (<em>Dbp</em>). In this line of mice, firefly luciferase is expressed from the <em>Dbp</em> locus in a <em>Cre</em>-recombinase- dependent manner, allowing assessment of bioluminescence rhythms in specific cellular populations. A mouse line in which luciferase expression was <em>Cre</em>-independent was also generated. The <em>Dbp</em> reporter alleles do not alter <em>Dbp</em> gene expression rhythms in liver or circadian locomotor activity rhythms. <em>In vitro</em> and <em>in vivo</em> studies show the utility of the reporter alleles for monitoring rhythmicity. Our studies reveal cell-type specific characteristics of rhythms among neuronal populations within the suprachiasmatic nuclei <em>in vitro</em>. <em>In vivo</em> studies show stable <em>Dbp</em>-driven bioluminescence rhythms in the liver of <em>Albumin-Cre;Dbp<sup>KI/+</sup></em> “liver reporter” mice. After a shift of the lighting schedule, locomotor activity achieved the proper phase relationship with the new lighting cycle more rapidly than hepatic bioluminescence did. As previously shown, restricting food access to the daytime altered the phase of hepatic rhythmicity. Our model allowed assessment of the rate of recovery from misalignment once animals were provided with food <em>ad libitum</em>. These studies provide clear evidence for circadian misalignment following environmental perturbations and reveal the utility of this model for minimally invasive, longitudinal monitoring of rhythmicity from specific mouse tissues.</p>
dc.identifier.submissionpathfaculty_pubs/2023
dc.contributor.departmentWeaver Lab
dc.contributor.departmentNeuroNexus Institute
dc.contributor.departmentBiochemistry and Molecular Pharmacology
dc.contributor.departmentMolecular, Cell and Cancer Biology
dc.contributor.departmentGraduate School of Biomedical Sciences, Neuroscience Program
dc.contributor.departmentNeurobiology
dc.contributor.studentCiearra B. Smith
dc.description.thesisprogramNeuroscience


Files in this item

Thumbnail
Name:
2021.04.04.438413v1.full.pdf
Size:
5.904Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record

The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Except where otherwise noted, this item's license is described as The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.