Simultaneous profiling of multiple chromatin proteins in the same cells [preprint]
UMass Chan AffiliationsGarber Lab
Graduate School of Biomedical Sciences
Program in Systems Biology
Program in Bioinformatics and Integrative Biology
Department of Molecular, Cell, and Cancer Biology
Amino Acids, Peptides, and Proteins
Nucleic Acids, Nucleotides, and Nucleosides
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AbstractMethods derived from CUT&RUN and CUT&Tag enable genome-wide mapping of the localization of proteins on chromatin from as few as one cell. These and other mapping approaches focus on one protein at a time, preventing direct measurements of colocalization of different chromatin proteins in the same cells and requiring prioritization of targets where samples are limiting. Here we describe multi-CUT&Tag, an adaptation of CUT&Tag that overcomes these hurdles by using antibody-specific barcodes to simultaneously map multiple proteins in the same cells. Highly specific multi-CUT&Tag maps of histone marks and RNA Polymerase II uncovered sites of co-localization in the same cells, active and repressed genes, and candidate cis-regulatory elements. Single-cell multi-CUT&Tag profiling facilitated identification of distinct cell types from a mixed population and characterization of cell type-specific chromatin architecture. In sum, multi-CUT&Tag increases the information content per cell of epigenomic maps, facilitating direct analysis of the interplay of different proteins on chromatin.
bioRxiv 2021.04.27.441642; doi: https://doi.org/10.1101/2021.04.27.441642. Link to preprint on bioRxiv.
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/29837
This article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.
Now published in Molecular Cell, doi: 10.1016/j.molcel.2021.09.019. Link to article on publisher's site