Simultaneous profiling of multiple chromatin proteins in the same cells [preprint]
UMass Chan Affiliations
Garber LabGraduate School of Biomedical Sciences
Program in Systems Biology
Program in Bioinformatics and Integrative Biology
Department of Molecular, Cell, and Cancer Biology
Document Type
PreprintPublication Date
2021-04-28Keywords
Genomicschromatin proteins
multi-CUT&Tag mapping
Amino Acids, Peptides, and Proteins
Bioinformatics
Cell Biology
Genomics
Molecular Biology
Nucleic Acids, Nucleotides, and Nucleosides
Metadata
Show full item recordAbstract
Methods derived from CUT&RUN and CUT&Tag enable genome-wide mapping of the localization of proteins on chromatin from as few as one cell. These and other mapping approaches focus on one protein at a time, preventing direct measurements of colocalization of different chromatin proteins in the same cells and requiring prioritization of targets where samples are limiting. Here we describe multi-CUT&Tag, an adaptation of CUT&Tag that overcomes these hurdles by using antibody-specific barcodes to simultaneously map multiple proteins in the same cells. Highly specific multi-CUT&Tag maps of histone marks and RNA Polymerase II uncovered sites of co-localization in the same cells, active and repressed genes, and candidate cis-regulatory elements. Single-cell multi-CUT&Tag profiling facilitated identification of distinct cell types from a mixed population and characterization of cell type-specific chromatin architecture. In sum, multi-CUT&Tag increases the information content per cell of epigenomic maps, facilitating direct analysis of the interplay of different proteins on chromatin.Source
bioRxiv 2021.04.27.441642; doi: https://doi.org/10.1101/2021.04.27.441642. Link to preprint on bioRxiv.
DOI
10.1101/2021.04.27.441642Permanent Link to this Item
http://hdl.handle.net/20.500.14038/29837Notes
This article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.
Related Resources
Now published in Molecular Cell, doi: 10.1016/j.molcel.2021.09.019. Link to article on publisher's site
Rights
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.Distribution License
http://creativecommons.org/licenses/by-nc-nd/4.0/ae974a485f413a2113503eed53cd6c53
10.1101/2021.04.27.441642
Scopus Count
Except where otherwise noted, this item's license is described as The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.