QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy [preprint]
UMass Chan Affiliations
Program in Molecular MedicineDocument Type
PreprintPublication Date
2021-01-27Keywords
light microscopyreproducibility
standards
quality
reproducibility
Biochemistry, Biophysics, and Structural Biology
Bioimaging and Biomedical Optics
Bioinformatics
Laboratory and Basic Science Research
Research Methods in Life Sciences
Metadata
Show full item recordAbstract
In April 2020, the QUality Assessment and REProducibility for Instruments and Images in Light Microscopy (QUAREP-LiMi) initiative was formed. This initiative comprises imaging scientists from academia and industry who share a common interest in achieving a better understanding of the performance and limitations of microscopes and improved quality control (QC) in light microscopy. The ultimate goal of the QUAREP-LiMi initiative is to establish a set of common QC standards, guidelines, metadata models, and tools, including detailed protocols, with the ultimate aim of improving reproducible advances in scientific research. This White Paper 1) summarizes the major obstacles identified in the field that motivated the launch of the QUAREP-LiMi initiative; 2) identifies the urgent need to address these obstacles in a grassroots manner, through a community of stakeholders including, researchers, imaging scientists, bioimage analysts, bioimage informatics developers, corporate partners, funding agencies, standards organizations, scientific publishers, and observers of such; 3) outlines the current actions of the QUAREP-LiMi initiative, and 4) proposes future steps that can be taken to improve the dissemination and acceptance of the proposed guidelines to manage QC. To summarize, the principal goal of the QUAREP-LiMi initiative is to improve the overall quality and reproducibility of light microscope image data by introducing broadly accepted standard practices and accurately captured image data metrics.Source
Nelson G, Boehm U, Bagley S, Bajcsy P, Bischof J, Brown CM, Dauphin A, Dobbie IM, Eriksson JE, Faklaris O, Fernandez-Rodriguez J. QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy. arXiv preprint arXiv:2101.09153. 2021 Jan 21. Link to preprint on arXiv.
DOI
10.1111/jmi.13041Permanent Link to this Item
http://hdl.handle.net/20.500.14038/29883Notes
This article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.
The PDF available for download is Version 2 of this preprint. The complete version history of this preprint is available at arXiv.org.
Full author list omitted for brevity. For the full list of authors, see article.
Related Resources
Now published in Journal of Microscopy, doi:10.1111/jmi.13041.