Comprehensive Structure and Functional Adaptations of the Yeast Nuclear Pore Complex [preprint]
AuthorsAkey, Christopher W.
Rout, Michael P.
UMass Chan AffiliationsDepartment of Biochemistry and Molecular Pharmacology
KeywordsNuclear pore complex
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AbstractNuclear Pore Complexes (NPCs) mediate the nucleocytoplasmic transport of macromolecules. Here we provide a structure of the yeast NPC in which the inner ring is resolved by cryo-EM at - helical resolution to show how flexible connectors tie together different structural and functional layers in the spoke. These connectors are targets for phosphorylation and regulated disassembly in cells with an open mitosis. Moreover, some nucleoporin pairs and karyopherins have similar interaction motifs, which suggests an evolutionary and mechanistic link between assembly and transport. We also provide evidence for three major NPC variants that foreshadow functional specializations at the nuclear periphery. Cryo-electron tomography extended these studies to provide a comprehensive model of the in situ NPC with a radially-expanded inner ring. Our model reveals novel features of the central transporter and nuclear basket, suggests a role for the lumenal ring in restricting dilation and highlights the structural plasticity required for transport by the NPC.
bioRxiv 2021.10.29.466335; doi: https://doi.org/10.1101/2021.10.29.466335. Link to preprint on bioRxiv.
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/29892
This article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.Full author list omitted for brevity. For the full list of authors, see article.
The PDF available for download is Version 2 of this preprint. The complete version history of this preprint is available at https://doi.org/10.1101/2021.10.29.466335.