piRNA-independent transposon silencing by the Drosophila THO complex
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UMass Chan Affiliations
Graduate School of Biomedical SciencesProgram in Bioinformatics and Integrative Biology
Program in Molecular Medicine
Document Type
Journal ArticlePublication Date
2021-09-27Keywords
H3K4me2H3K9me3
Piwi
THO
Thoc7
germline development
piRNA
transcriptional silencing
transposon silencing
Amino Acids, Peptides, and Proteins
Cell and Developmental Biology
Nucleic Acids, Nucleotides, and Nucleosides
Metadata
Show full item recordAbstract
piRNAs guide Piwi/Panoramix-dependent H3K9me3 chromatin modification and transposon silencing during Drosophila germline development. The THO RNA export complex is composed of Hpr1, Tho2, and Thoc5-7. Null thoc7 mutations, which displace Thoc5 and Thoc6 from a Tho2-Hpr1 subcomplex, reduce expression of a subset of germline piRNAs and increase transposon expression, suggesting that THO silences transposons by promoting piRNA biogenesis. Here, we show that the thoc7-null mutant combination increases transposon transcription but does not reduce anti-sense piRNAs targeting half of the transcriptionally activated transposon families. These mutations also fail to reduce piRNA-guided H3K9me3 chromatin modification or block Panoramix-dependent silencing of a reporter transgene, and unspliced transposon transcripts co-precipitate with THO through a Piwi- and Panoramix-independent mechanism. Mutations in piwi also dominantly enhance germline defects associated with thoc7-null alleles. THO thus functions in a piRNA-independent transposon-silencing pathway, which acts cooperatively with Piwi to support germline development.Source
Zhang G, Yu T, Parhad SS, Ho S, Weng Z, Theurkauf WE. piRNA-independent transposon silencing by the Drosophila THO complex. Dev Cell. 2021 Sep 27;56(18):2623-2635.e5. doi: 10.1016/j.devcel.2021.08.021. Epub 2021 Sep 20. PMID: 34547226; PMCID: PMC8567991. Link to article on publisher's site
DOI
10.1016/j.devcel.2021.08.021Permanent Link to this Item
http://hdl.handle.net/20.500.14038/29918PubMed ID
34547226Related Resources
ae974a485f413a2113503eed53cd6c53
10.1016/j.devcel.2021.08.021