Authors
Kapinas, KristinaGrandy, Rodrigo
Ghule, Prachi N.
Medina, Ricardo F.
Becker, Klaus A.
Pardee, Arthur B.
Zaidi, Sayyed K.
Lian, Jane B.
Stein, Janet L.
Van Wijnen, Andre J.
Stein, Gary S.
UMass Chan Affiliations
Department of Cell and Developmental BiologyDocument Type
Journal ArticlePublication Date
2013-01-01Keywords
AnimalsCell Cycle
Cell Nucleus
Embryonic Stem Cells
Gene Expression Regulation
Genes, myc
Histones
Humans
MicroRNAs
Pluripotent Stem Cells
Cell Biology
Cellular and Molecular Physiology
Metadata
Show full item recordAbstract
Human embryonic stem cells (hESCs) and induced pluripotent stem cells proliferate rapidly and divide symmetrically producing equivalent progeny cells. In contrast, lineage committed cells acquire an extended symmetrical cell cycle. Self-renewal of tissue-specific stem cells is sustained by asymmetric cell division where one progeny cell remains a progenitor while the partner progeny cell exits the cell cycle and differentiates. There are three principal contexts for considering the operation and regulation of the pluripotent cell cycle: temporal, regulatory, and structural. The primary temporal context that the pluripotent self-renewal cell cycle of hESCs is a short G1 period without reducing periods of time allocated to S phase, G2, and mitosis. The rules that govern proliferation in hESCs remain to be comprehensively established. However, several lines of evidence suggest a key role for the naive transcriptome of hESCs, which is competent to stringently regulate the embryonic stem cell (ESC) cell cycle. This supports the requirements of pluripotent cells to self-propagate while suppressing expression of genes that confer lineage commitment and/or tissue specificity. However, for the first time, we consider unique dimensions to the architectural organization and assembly of regulatory machinery for gene expression in nuclear microenviornments that define parameters of pluripotency. From both fundamental biological and clinical perspectives, understanding control of the abbreviated ESC cycle can provide options to coordinate control of proliferation versus differentiation. Wound healing, tissue engineering, and cell-based therapy to mitigate developmental aberrations illustrate applications that benefit from knowledge of the biology of the pluripotent cell cycle.Source
J Cell Physiol. 2013 Jan;228(1):9-20. doi: 10.1002/jcp.24104. Link to article on publisher's siteDOI
10.1002/jcp.24104Permanent Link to this Item
http://hdl.handle.net/20.500.14038/29990PubMed ID
22552993Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1002/jcp.24104