CD4+ T Cells Provide Intermolecular Help To Generate Robust Antibody Responses in Vaccinia Virus-Vaccinated Humans
Authors
Yin, LiusongCalvo-Calle, J. Mauricio
Cruz, John Jr.
Newman, Frances K.
Frey, Sharon E.
Ennis, Francis A.
Stern, Lawrence J.
UMass Chan Affiliations
Center for Infectious Disease and Vaccine ResearchDepartment of Pathology
Department of Medicine, Division of Infectious Diseases and Immunology
Document Type
Journal ArticlePublication Date
2013-06-15Keywords
CD4-Positive T-LymphocytesVaccinia virus
Antibodies, Heterophile
Immunology and Infectious Disease
Metadata
Show full item recordAbstract
Immunization with vaccinia virus elicits a protective Ab response that is almost completely CD4+ T cell dependent. A recent study in a rodent model observed a deterministic linkage between Ab and CD4+ T cell responses to particular vaccinia virus proteins suggesting that CD4+ T cell help is preferentially provided to B cells with the same protein specificity (Sette et al. 2008. Immunity 28: 847-858). However, a causal linkage between Ab and CD4+ T cell responses to vaccinia or any other large pathogen in humans has yet to be done. In this study, we measured the Ab and CD4+ T cell responses against four vaccinia viral proteins (A27L, A33R, B5R, and L1R) known to be strongly targeted by humoral and cellular responses induced by vaccinia virus vaccination in 90 recently vaccinated and 7 long-term vaccinia-immunized human donors. Our data indicate that there is no direct linkage between Ab and CD4+ T cell responses against each individual protein in both short-term and long-term immunized donors. Together with the observation that the presence of immune responses to these four proteins is linked together within donors, our data suggest that in vaccinia-immunized humans, individual viral proteins are not the primary recognition unit of CD4+ T cell help for B cells. Therefore, we have for the first time, to our knowledge, shown evidence that CD4+ T cells provide intermolecular (also known as noncognate or heterotypic) help to generate robust Ab responses against four vaccinia viral proteins in humans.Source
J Immunol. 2013 Jun 15;190(12):6023-33. doi: 10.4049/jimmunol.1202523. Link to article on publisher's siteDOI
10.4049/jimmunol.1202523Permanent Link to this Item
http://hdl.handle.net/20.500.14038/29999PubMed ID
23667112Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.4049/jimmunol.1202523