Differential requirement for SUB1 in chromosomal and plasmid double-strand DNA break repair
Volkert, Michael R.
UMass Chan AffiliationsDepartment of Microbiology and Physiological Systems
Document TypeJournal Article
KeywordsAmino Acids, Peptides, and Proteins
Enzymes and Coenzymes
Genetics and Genomics
MetadataShow full item record
AbstractNon homologous end joining (NHEJ) is an important process that repairs double strand DNA breaks (DSBs) in eukaryotic cells. Cells defective in NHEJ are unable to join chromosomal breaks. Two different NHEJ assays are typically used to determine the efficiency of NHEJ. One requires NHEJ of linearized plasmid DNA transformed into the test organism; the other requires NHEJ of a single chromosomal break induced either by HO endonuclease or the I-SceI restriction enzyme. These two assays are generally considered equivalent and rely on the same set of NHEJ genes. PC4 is an abundant DNA binding protein that has been suggested to stimulate NHEJ. Here we tested the role of PC4's yeast homolog SUB1 in repair of DNA double strand breaks using different assays. We found SUB1 is required for NHEJ repair of DSBs in plasmid DNA, but not in chromosomal DNA. Our results suggest that these two assays, while similar are not equivalent and that repair of plasmid DNA requires additional factor(s) that are not required for NHEJ repair of chromosomal double-strand DNA breaks. Possible roles for Sub1 proteins in NHEJ of plasmid DNA are discussed.
PLoS One. 2013;8(3):e58015. doi: 10.1371/journal.pone.0058015. Epub 2013 Mar 12. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/30014
RightsCopyright: 2013 Yu, Volkert. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.