Show simple item record

dc.contributor.authorO'Bryan, Joel M.
dc.contributor.authorWoda, Marcia
dc.contributor.authorCo, Mary Dawn T.
dc.contributor.authorMathew, Anuja
dc.contributor.authorRothman, Alan
dc.date2022-08-11T08:08:29.000
dc.date.accessioned2022-08-23T15:56:59Z
dc.date.available2022-08-23T15:56:59Z
dc.date.issued2013-08-26
dc.date.submitted2014-03-11
dc.identifier.citationO'Bryan JM, Woda M, Co M, Mathew A, Rothman AL. Telomere length dynamics in human memory T cells specific for viruses causing acute or latent infections. Immun Ageing. 2013 Aug 26;10(1):37. doi: 10.1186/1742-4933-10-37. <a href="http://dx.doi.org/10.1186/1742-4933-10-37">Link to article on publisher's site</a>
dc.identifier.issn1742-4933 (Linking)
dc.identifier.doi10.1186/1742-4933-10-37
dc.identifier.pmid23971624
dc.identifier.urihttp://hdl.handle.net/20.500.14038/30090
dc.description.abstractBACKGROUND: Declining telomere length (TL) is associated with T cell senescence. While TL in naive and memory T cells declines with increasing age, there is limited data on TL dynamics in virus-specific memory CD4+ T cells in healthy adults. We combined BrdU-labeling of virus-stimulated T cells followed with flow cytometry-fluorescent in situ hybridization for TL determination. We analyzed TL in T cells specific for several virus infections: non-recurring acute (vaccinia virus, VACV), recurring-acute (influenza A virus, IAV), and reactivating viruses (varicella-zoster virus, VZV, and cytomegalovirus, CMV) in 10 healthy subjects. Additionally, five subjects provided multiple blood samples separated by up to 10 years. RESULTS: VACV- and CMV-specific T cells had longer average TL than IAV-specific CD4+ T cells. Although most virus-specific cells were CD45RA-, we observed a minor population of BrdU+ CD45RA+ T cells characterized by long telomeres. Longitudinal analysis demonstrated a slow decline in average TL in virus-specific T cells. However, in one subject, VZV reactivation led to an increase in average TL in VZV-specific memory T cells, suggesting a conversion of longer TL cells from the naive T cell repertoire. CONCLUSIONS: TLs in memory CD4+ T cells in otherwise healthy adults are heterogeneous and follow distinct virus-specific kinetics. These findings suggests that the distribution of TL and the creation and maintenance of long TL memory T cells could be important for the persistence of long-lived T cell memory.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=23971624&dopt=Abstract">Link to Article in PubMed</a>
dc.rightsCopyright 2013 O'Bryan et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<a href="http://creativecommons.org/licenses/by/2.0">http://creativecommons.org/licenses/by/2.0</a>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.subjectAgeing
dc.subjectTelomere
dc.subjectT cell memory
dc.subjectCD45RA
dc.subjectFlowFISH
dc.subjectInfluenza A virus
dc.subjectCytomegalovirus
dc.subjectVaccinia virus
dc.subjectVaricella zoster virus
dc.subjectBrdU labeling
dc.subjectImmunology of Infectious Disease
dc.titleTelomere length dynamics in human memory T cells specific for viruses causing acute or latent infections
dc.typeJournal Article
dc.source.journaltitleImmunity and ageing : I and A
dc.source.volume10
dc.source.issue1
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1325&amp;context=faculty_pubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/faculty_pubs/326
dc.identifier.contextkey5319141
refterms.dateFOA2022-08-23T15:56:59Z
html.description.abstract<p>BACKGROUND: Declining telomere length (TL) is associated with T cell senescence. While TL in naive and memory T cells declines with increasing age, there is limited data on TL dynamics in virus-specific memory CD4+ T cells in healthy adults. We combined BrdU-labeling of virus-stimulated T cells followed with flow cytometry-fluorescent in situ hybridization for TL determination. We analyzed TL in T cells specific for several virus infections: non-recurring acute (vaccinia virus, VACV), recurring-acute (influenza A virus, IAV), and reactivating viruses (varicella-zoster virus, VZV, and cytomegalovirus, CMV) in 10 healthy subjects. Additionally, five subjects provided multiple blood samples separated by up to 10 years.</p> <p>RESULTS: VACV- and CMV-specific T cells had longer average TL than IAV-specific CD4+ T cells. Although most virus-specific cells were CD45RA-, we observed a minor population of BrdU+ CD45RA+ T cells characterized by long telomeres. Longitudinal analysis demonstrated a slow decline in average TL in virus-specific T cells. However, in one subject, VZV reactivation led to an increase in average TL in VZV-specific memory T cells, suggesting a conversion of longer TL cells from the naive T cell repertoire.</p> <p>CONCLUSIONS: TLs in memory CD4+ T cells in otherwise healthy adults are heterogeneous and follow distinct virus-specific kinetics. These findings suggests that the distribution of TL and the creation and maintenance of long TL memory T cells could be important for the persistence of long-lived T cell memory.</p>
dc.identifier.submissionpathfaculty_pubs/326
dc.contributor.departmentDepartment of Medicine, Division of Infectious Diseases and Immunology
dc.source.pages37


Files in this item

Thumbnail
Name:
Immunity_and_aging_o_bryan_174 ...
Size:
3.065Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record