Telomere length dynamics in human memory T cells specific for viruses causing acute or latent infections
dc.contributor.author | O'Bryan, Joel M. | |
dc.contributor.author | Woda, Marcia | |
dc.contributor.author | Co, Mary Dawn T. | |
dc.contributor.author | Mathew, Anuja | |
dc.contributor.author | Rothman, Alan | |
dc.date | 2022-08-11T08:08:29.000 | |
dc.date.accessioned | 2022-08-23T15:56:59Z | |
dc.date.available | 2022-08-23T15:56:59Z | |
dc.date.issued | 2013-08-26 | |
dc.date.submitted | 2014-03-11 | |
dc.identifier.citation | O'Bryan JM, Woda M, Co M, Mathew A, Rothman AL. Telomere length dynamics in human memory T cells specific for viruses causing acute or latent infections. Immun Ageing. 2013 Aug 26;10(1):37. doi: 10.1186/1742-4933-10-37. <a href="http://dx.doi.org/10.1186/1742-4933-10-37">Link to article on publisher's site</a> | |
dc.identifier.issn | 1742-4933 (Linking) | |
dc.identifier.doi | 10.1186/1742-4933-10-37 | |
dc.identifier.pmid | 23971624 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/30090 | |
dc.description.abstract | BACKGROUND: Declining telomere length (TL) is associated with T cell senescence. While TL in naive and memory T cells declines with increasing age, there is limited data on TL dynamics in virus-specific memory CD4+ T cells in healthy adults. We combined BrdU-labeling of virus-stimulated T cells followed with flow cytometry-fluorescent in situ hybridization for TL determination. We analyzed TL in T cells specific for several virus infections: non-recurring acute (vaccinia virus, VACV), recurring-acute (influenza A virus, IAV), and reactivating viruses (varicella-zoster virus, VZV, and cytomegalovirus, CMV) in 10 healthy subjects. Additionally, five subjects provided multiple blood samples separated by up to 10 years. RESULTS: VACV- and CMV-specific T cells had longer average TL than IAV-specific CD4+ T cells. Although most virus-specific cells were CD45RA-, we observed a minor population of BrdU+ CD45RA+ T cells characterized by long telomeres. Longitudinal analysis demonstrated a slow decline in average TL in virus-specific T cells. However, in one subject, VZV reactivation led to an increase in average TL in VZV-specific memory T cells, suggesting a conversion of longer TL cells from the naive T cell repertoire. CONCLUSIONS: TLs in memory CD4+ T cells in otherwise healthy adults are heterogeneous and follow distinct virus-specific kinetics. These findings suggests that the distribution of TL and the creation and maintenance of long TL memory T cells could be important for the persistence of long-lived T cell memory. | |
dc.language.iso | en_US | |
dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=23971624&dopt=Abstract">Link to Article in PubMed</a> | |
dc.rights | Copyright 2013 O'Bryan et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<a href="http://creativecommons.org/licenses/by/2.0">http://creativecommons.org/licenses/by/2.0</a>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |
dc.subject | Ageing | |
dc.subject | Telomere | |
dc.subject | T cell memory | |
dc.subject | CD45RA | |
dc.subject | FlowFISH | |
dc.subject | Influenza A virus | |
dc.subject | Cytomegalovirus | |
dc.subject | Vaccinia virus | |
dc.subject | Varicella zoster virus | |
dc.subject | BrdU labeling | |
dc.subject | Immunology of Infectious Disease | |
dc.title | Telomere length dynamics in human memory T cells specific for viruses causing acute or latent infections | |
dc.type | Journal Article | |
dc.source.journaltitle | Immunity and ageing : I and A | |
dc.source.volume | 10 | |
dc.source.issue | 1 | |
dc.identifier.legacyfulltext | https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1325&context=faculty_pubs&unstamped=1 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/faculty_pubs/326 | |
dc.identifier.contextkey | 5319141 | |
refterms.dateFOA | 2022-08-23T15:56:59Z | |
html.description.abstract | <p>BACKGROUND: Declining telomere length (TL) is associated with T cell senescence. While TL in naive and memory T cells declines with increasing age, there is limited data on TL dynamics in virus-specific memory CD4+ T cells in healthy adults. We combined BrdU-labeling of virus-stimulated T cells followed with flow cytometry-fluorescent in situ hybridization for TL determination. We analyzed TL in T cells specific for several virus infections: non-recurring acute (vaccinia virus, VACV), recurring-acute (influenza A virus, IAV), and reactivating viruses (varicella-zoster virus, VZV, and cytomegalovirus, CMV) in 10 healthy subjects. Additionally, five subjects provided multiple blood samples separated by up to 10 years.</p> <p>RESULTS: VACV- and CMV-specific T cells had longer average TL than IAV-specific CD4+ T cells. Although most virus-specific cells were CD45RA-, we observed a minor population of BrdU+ CD45RA+ T cells characterized by long telomeres. Longitudinal analysis demonstrated a slow decline in average TL in virus-specific T cells. However, in one subject, VZV reactivation led to an increase in average TL in VZV-specific memory T cells, suggesting a conversion of longer TL cells from the naive T cell repertoire.</p> <p>CONCLUSIONS: TLs in memory CD4+ T cells in otherwise healthy adults are heterogeneous and follow distinct virus-specific kinetics. These findings suggests that the distribution of TL and the creation and maintenance of long TL memory T cells could be important for the persistence of long-lived T cell memory.</p> | |
dc.identifier.submissionpath | faculty_pubs/326 | |
dc.contributor.department | Department of Medicine, Division of Infectious Diseases and Immunology | |
dc.source.pages | 37 |