HOX antisense lincRNA HOXA-AS2 is an apoptosis repressor in all trans retinoic acid treated NB4 promyelocytic leukemia cells
UMass Chan Affiliations
Department of PediatricsDocument Type
Journal ArticlePublication Date
2013-05-03Keywords
Homeodomain ProteinsRNA, Long Untranslated
Apoptosis
Apoptosis Regulatory Proteins
Leukemia, Promyelocytic, Acute
Cancer Biology
Hemic and Lymphatic Diseases
Neoplasms
Metadata
Show full item recordAbstract
HOXA cluster antisense RNA 2 (HOXA-AS2) is a long non-coding RNA located between the HOXA3 and HOXA4 genes in the HOXA cluster. Its transcript is expressed in NB4 promyelocytic leukemia cells and human peripheral blood neutrophils, and expression is increased in NB4 cells treated with all trans retinoic acid (ATRA). Knockdown of HOXA-AS2 expression by transduced shRNA decreases the number of viable cells and increases the proportion of apoptotic cells, measured by annexin V binding and by activity and cleavage of caspases-3, -8, and -9. The increase in death of HOXA-AS2 knockdown cells was accompanied by an elevated TNF-related apoptosis-inducing ligand (TRAIL) levels, but ATRA-induced NB4 cells treated with TRAIL did show an increase in HOXA-AS2 expression. These results demonstrate that ATRA induction of HOXA-AS2 suppresses ATRA-induced apoptosis, possibly through a TRAIL-mediated pathway. HOXA-AS2-mediated negative regulation thus contributes to the fine-tuning of apoptosis during ATRA-induced myeloid differentiation in NB4 cells. J. Cell. Biochem. (c) 2013 Wiley Periodicals, Inc.Source
J Cell Biochem. 2013 May 3. doi: 10.1002/jcb.24586. Link to article on publisher's siteDOI
10.1002/jcb.24586Permanent Link to this Item
http://hdl.handle.net/20.500.14038/30094PubMed ID
23649634Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1002/jcb.24586