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dc.contributor.authorZhao, Hang
dc.contributor.authorZhang, Xueqing
dc.contributor.authorFrazao, Josias Brito
dc.contributor.authorCondino-Neto, Antonio
dc.contributor.authorNewburger, Peter E.
dc.date2022-08-11T08:08:29.000
dc.date.accessioned2022-08-23T15:57:00Z
dc.date.available2022-08-23T15:57:00Z
dc.date.issued2013-05-03
dc.date.submitted2013-06-05
dc.identifier.citationJ Cell Biochem. 2013 May 3. doi: 10.1002/jcb.24586. <a href="http://dx.doi.org/10.1002/jcb.24586">Link to article on publisher's site</a>
dc.identifier.issn0730-2312 (Linking)
dc.identifier.doi10.1002/jcb.24586
dc.identifier.pmid23649634
dc.identifier.urihttp://hdl.handle.net/20.500.14038/30094
dc.description.abstractHOXA cluster antisense RNA 2 (HOXA-AS2) is a long non-coding RNA located between the HOXA3 and HOXA4 genes in the HOXA cluster. Its transcript is expressed in NB4 promyelocytic leukemia cells and human peripheral blood neutrophils, and expression is increased in NB4 cells treated with all trans retinoic acid (ATRA). Knockdown of HOXA-AS2 expression by transduced shRNA decreases the number of viable cells and increases the proportion of apoptotic cells, measured by annexin V binding and by activity and cleavage of caspases-3, -8, and -9. The increase in death of HOXA-AS2 knockdown cells was accompanied by an elevated TNF-related apoptosis-inducing ligand (TRAIL) levels, but ATRA-induced NB4 cells treated with TRAIL did show an increase in HOXA-AS2 expression. These results demonstrate that ATRA induction of HOXA-AS2 suppresses ATRA-induced apoptosis, possibly through a TRAIL-mediated pathway. HOXA-AS2-mediated negative regulation thus contributes to the fine-tuning of apoptosis during ATRA-induced myeloid differentiation in NB4 cells. J. Cell. Biochem. (c) 2013 Wiley Periodicals, Inc.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=23649634&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1002/jcb.24586
dc.subjectHomeodomain Proteins
dc.subjectRNA, Long Untranslated
dc.subjectApoptosis
dc.subjectApoptosis Regulatory Proteins
dc.subjectLeukemia, Promyelocytic, Acute
dc.subjectCancer Biology
dc.subjectHemic and Lymphatic Diseases
dc.subjectNeoplasms
dc.titleHOX antisense lincRNA HOXA-AS2 is an apoptosis repressor in all trans retinoic acid treated NB4 promyelocytic leukemia cells
dc.typeJournal Article
dc.source.journaltitleJournal of cellular biochemistry
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/faculty_pubs/33
dc.identifier.contextkey4199963
html.description.abstract<p>HOXA cluster antisense RNA 2 (HOXA-AS2) is a long non-coding RNA located between the HOXA3 and HOXA4 genes in the HOXA cluster. Its transcript is expressed in NB4 promyelocytic leukemia cells and human peripheral blood neutrophils, and expression is increased in NB4 cells treated with all trans retinoic acid (ATRA). Knockdown of HOXA-AS2 expression by transduced shRNA decreases the number of viable cells and increases the proportion of apoptotic cells, measured by annexin V binding and by activity and cleavage of caspases-3, -8, and -9. The increase in death of HOXA-AS2 knockdown cells was accompanied by an elevated TNF-related apoptosis-inducing ligand (TRAIL) levels, but ATRA-induced NB4 cells treated with TRAIL did show an increase in HOXA-AS2 expression. These results demonstrate that ATRA induction of HOXA-AS2 suppresses ATRA-induced apoptosis, possibly through a TRAIL-mediated pathway. HOXA-AS2-mediated negative regulation thus contributes to the fine-tuning of apoptosis during ATRA-induced myeloid differentiation in NB4 cells. J. Cell. Biochem. (c) 2013 Wiley Periodicals, Inc.</p>
dc.identifier.submissionpathfaculty_pubs/33
dc.contributor.departmentDepartment of Pediatrics


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