Show simple item record

dc.contributor.authorKearns, Nicola A.
dc.contributor.authorGenga, Ryan M. J.
dc.contributor.authorEnuameh, Metewo Selase
dc.contributor.authorGarber, Manuel
dc.contributor.authorWolfe, Scot A.
dc.contributor.authorMaehr, Rene
dc.date2022-08-11T08:08:29.000
dc.date.accessioned2022-08-23T15:57:02Z
dc.date.available2022-08-23T15:57:02Z
dc.date.issued2014-01-01
dc.date.submitted2014-03-27
dc.identifier.citation<p>Kearns NA, Genga RM, Enuameh MS, Garber M, Wolfe SA, Maehr R. Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi:10.1242/dev.103341. <a href="http://dx.doi.org/10.1242/dev.103341">Link to article on publisher's site</a></p>
dc.identifier.issn0950-1991 (Linking)
dc.identifier.doi10.1242/dev.103341
dc.identifier.pmid24346702
dc.identifier.urihttp://hdl.handle.net/20.500.14038/30102
dc.description.abstractThe identification of the trans-acting factors and cis-regulatory modules that are involved in human pluripotent stem cell (hPSC) maintenance and differentiation is necessary to dissect the operating regulatory networks in these processes and thereby identify nodes where signal input will direct desired cell fate decisions in vitro or in vivo. To deconvolute these networks, we established a method to influence the differentiation state of hPSCs with a CRISPR-associated catalytically inactive dCas9 fused to an effector domain. In human embryonic stem cells, we find that the dCas9 effectors can exert positive or negative regulation on the expression of developmentally relevant genes, which can influence cell differentiation status when impinging on a key node in the regulatory network that governs the cell state. This system provides a platform for the interrogation of the underlying regulators governing specific differentiation decisions, which can then be employed to direct cellular differentiation down desired pathways.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=24346702&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3865759/
dc.subjectAmino Acid Sequence
dc.subjectCaspase 9
dc.subjectCell Differentiation
dc.subjectCell Lineage
dc.subjectClustered Regularly Interspaced Short Palindromic Repeats
dc.subjectDNA-Binding Proteins
dc.subjectEmbryonic Stem Cells
dc.subjectGene Regulatory Networks
dc.subjectHEK293 Cells
dc.subjectHumans
dc.subjectMolecular Sequence Data
dc.subjectOctamer Transcription Factor-3
dc.subjectPluripotent Stem Cells
dc.subjectSOXF Transcription Factors
dc.subjectTranscription, Genetic
dc.subjectTranscriptional Activation
dc.subjectUMCCTS funding
dc.subjectCell Biology
dc.subjectCellular and Molecular Physiology
dc.subjectDevelopmental Biology
dc.subjectMolecular Genetics
dc.titleCas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells
dc.typeJournal Article
dc.source.journaltitleDevelopment (Cambridge, England)
dc.source.volume141
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/faculty_pubs/337
dc.identifier.contextkey5395153
html.description.abstract<p>The identification of the trans-acting factors and cis-regulatory modules that are involved in human pluripotent stem cell (hPSC) maintenance and differentiation is necessary to dissect the operating regulatory networks in these processes and thereby identify nodes where signal input will direct desired cell fate decisions in vitro or in vivo. To deconvolute these networks, we established a method to influence the differentiation state of hPSCs with a CRISPR-associated catalytically inactive dCas9 fused to an effector domain. In human embryonic stem cells, we find that the dCas9 effectors can exert positive or negative regulation on the expression of developmentally relevant genes, which can influence cell differentiation status when impinging on a key node in the regulatory network that governs the cell state. This system provides a platform for the interrogation of the underlying regulators governing specific differentiation decisions, which can then be employed to direct cellular differentiation down desired pathways.</p>
dc.identifier.submissionpathfaculty_pubs/337
dc.contributor.departmentBioinformatics and Integrative Biology
dc.contributor.departmentProgram in Gene Function and Expression
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentDiabetes Center of Excellence
dc.source.pages219-23


This item appears in the following Collection(s)

Show simple item record