Improved detection rate of cytogenetic abnormalities in chronic lymphocytic leukemia and other mature B-cell neoplasms with use of CpG-oligonucleotide DSP30 and interleukin 2 stimulation
Authors
Shi, MinCipollini, Matthew J.
Crowley-Bish, Patricia
Higgins, Anne W.
Yu, Hongbo
Miron, Patricia M.
Document Type
Journal ArticlePublication Date
2013-05-01Keywords
Chromosome AberrationsCytogenetics
Interleukin-2
Leukemia, Lymphocytic, Chronic, B-Cell
Oligonucleotides
Diagnosis
Hemic and Lymphatic Diseases
Oncology
Pathology
Metadata
Show full item recordAbstract
Detection of cytogenetic abnormalities requires successful culture of the clonal population to obtain metaphase chromosomes for study, and as such, has been hampered by low mitotic indices of mature B cells in culture. Our study presents data on the improved abnormality detection rate with the use of a CpG-oligonucleotide/interleukin 2 (OL/IL-2) culture protocol for mature B-cell neoplasms, including chronic lymphocytic leukemia (CLL) and non-CLL specimens. The increased detection rate of abnormalities, compared with unstimulated culture and traditional pokeweed mitogen culture, was statistically significant for both CLL and non-CLL neoplasms. For CLL specimens, our data also showed that for cytogenetically visible aberrations, OL/IL-2 was as, if not more, sensitive than detection with interphase fluorescence in situ hybridization (iFISH). Use of OL/IL-2 allowed a number of abnormalities to be detected, which were not covered by specific iFISH panels, especially balanced translocations. Therefore, OL/IL-2 stimulation improves diagnostic sensitivity and increases discovery rate of novel prognostic findings.Source
Am J Clin Pathol. 2013 May;139(5):662-9. doi: 10.1309/AJCP7G4VMYZJQVFI. Link to article on publisher's siteDOI
10.1309/AJCP7G4VMYZJQVFIPermanent Link to this Item
http://hdl.handle.net/20.500.14038/30112PubMed ID
23596118Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1309/AJCP7G4VMYZJQVFI