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    Generation of organized anterior foregut epithelia from pluripotent stem cells using small molecules

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    Authors
    Kearns, Nicola A.
    Genga, Ryan M. J.
    Ziller, Michael
    Kapinas, Kristina
    Peters, Heiko
    Brehm, Michael A.
    Meissner, Alexander
    Maehr, Rene
    UMass Chan Affiliations
    Program in Molecular Medicine
    Document Type
    Journal Article
    Publication Date
    2013-11-01
    Keywords
    Cell and Developmental Biology
    
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    Link to Full Text
    http://dx.doi.org/10.1016/j.scr.2013.06.007
    Abstract
    Anterior foregut endoderm (AFE) gives rise to therapeutically relevant cell types in tissues such as the esophagus, salivary glands, lung, thymus, parathyroid and thyroid. Despite its importance, reports describing the generation of AFE from pluripotent stem cells (PSCs) by directed differentiation have mainly focused on the Nkx2.1(+) lung and thyroid lineages. Here, we describe a novel protocol to derive a subdomain of AFE, identified by expression of Pax9, from PSCs using small molecules and defined media conditions. We generated a reporter PSC line for isolation and characterization of Pax9(+) AFE cells, which when transplanted in vivo, can form several distinct complex AFE-derived epithelia, including mucosal glands and stratified squamous epithelium. Finally, we show that the directed differentiation protocol can be used to generate AFE from human PSCs. Thus, this work both broadens the range of PSC-derived AFE tissues and creates a platform enabling the study of AFE disorders.
    Source
    Kearns NA, Genga RM, Ziller M, Kapinas K, Peters H, Brehm MA, Meissner A, Maehr R. Generation of organized anterior foregut epithelia from pluripotent stem cells using small molecules. Stem Cell Res. 2013 Nov;11(3):1003-12. doi:10.1016/j.scr.2013.06.007.Link to article on publisher's site
    DOI
    10.1016/j.scr.2013.06.007
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/30137
    PubMed ID
    23917481
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.scr.2013.06.007
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