Generation of organized anterior foregut epithelia from pluripotent stem cells using small molecules
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Authors
Kearns, Nicola A.Genga, Ryan M. J.
Ziller, Michael
Kapinas, Kristina
Peters, Heiko
Brehm, Michael A.
Meissner, Alexander
Maehr, Rene
UMass Chan Affiliations
Program in Molecular MedicineDocument Type
Journal ArticlePublication Date
2013-11-01Keywords
Cell and Developmental Biology
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Anterior foregut endoderm (AFE) gives rise to therapeutically relevant cell types in tissues such as the esophagus, salivary glands, lung, thymus, parathyroid and thyroid. Despite its importance, reports describing the generation of AFE from pluripotent stem cells (PSCs) by directed differentiation have mainly focused on the Nkx2.1(+) lung and thyroid lineages. Here, we describe a novel protocol to derive a subdomain of AFE, identified by expression of Pax9, from PSCs using small molecules and defined media conditions. We generated a reporter PSC line for isolation and characterization of Pax9(+) AFE cells, which when transplanted in vivo, can form several distinct complex AFE-derived epithelia, including mucosal glands and stratified squamous epithelium. Finally, we show that the directed differentiation protocol can be used to generate AFE from human PSCs. Thus, this work both broadens the range of PSC-derived AFE tissues and creates a platform enabling the study of AFE disorders.Source
Kearns NA, Genga RM, Ziller M, Kapinas K, Peters H, Brehm MA, Meissner A, Maehr R. Generation of organized anterior foregut epithelia from pluripotent stem cells using small molecules. Stem Cell Res. 2013 Nov;11(3):1003-12. doi:10.1016/j.scr.2013.06.007.Link to article on publisher's siteDOI
10.1016/j.scr.2013.06.007Permanent Link to this Item
http://hdl.handle.net/20.500.14038/30137PubMed ID
23917481Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/j.scr.2013.06.007