An amphiphilic degradable polymer/hydroxyapatite composite with enhanced handling characteristics promotes osteogenic gene expression in bone marrow stromal cells
UMass Chan AffiliationsDepartment of Cell and Developmental Biology
Department of Orthopedics and Physical Rehabilitation
Document TypeJournal Article
Equipment Failure Analysis
Gene Expression Regulation
Hydrophobic and Hydrophilic Interactions
Mesenchymal Stromal Cells
Cell and Developmental Biology
Molecular, Cellular, and Tissue Engineering
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AbstractElectrospun polymer/hydroxyapatite (HA) composites combining biodegradability with osteoconductivity are attractive for skeletal tissue engineering applications. However, most biodegradable polymers such as poly(lactic acid) (PLA) are hydrophobic and do not blend with adequate interfacial adhesion with HA, compromising the structural homogeneity, mechanical integrity and biological performance of the composite. To overcome this challenge, we combined a hydrophilic polyethylene glycol (PEG) block with poly(d,l-lactic acid) to improve the adhesion of the degradable polymer with HA. The amphiphilic triblock copolymer PLA-PEG-PLA (PELA) improved the stability of HA-PELA suspension at 25wt.% HA content, which was readily electrospun into HA-PELA composite scaffolds with uniform fiber dimensions. HA-PELA was highly extensible (failure strain>200% vs. 100 degrees for HA-PLA), and exhibited an 8-fold storage modulus increase (unlike deterioration for HA-PLA) upon hydration, owing to the favorable interaction between HA and PEG. HA-PELA also better promoted osteochondral lineage commitment of bone marrow stromal cells in unstimulated culture and supported far more potent osteogenic gene expression upon induction than HA-PLA. We demonstrate that the chemical incorporation of PEG is an effective strategy to improve the performance of degradable polymer/HA composites for bone tissue engineering applications.
Kutikov AB, Song J. An amphiphilic degradable polymer/hydroxyapatite composite with enhanced handling characteristics promotes osteogenic gene expression in bone marrow stromal cells. Acta Biomater. 2013 Sep;9(9):8354-64. doi:10.1016/j.actbio.2013.06.013. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/30139
First author Artem B. Kutikov is a doctoral student in the Cell Biology program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.