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dc.contributor.authorZhong, Li
dc.contributor.authorLi, Shaoyong
dc.contributor.authorLi, Mengxin
dc.contributor.authorXie, Jun
dc.contributor.authorZhang, Yu
dc.contributor.authorLee, Brendan
dc.contributor.authorBatshaw, Mark L.
dc.contributor.authorWilson, James M.
dc.contributor.authorGao, Guangping
dc.date2022-08-11T08:08:31.000
dc.date.accessioned2022-08-23T15:57:50Z
dc.date.available2022-08-23T15:57:50Z
dc.date.issued2013-09-01
dc.date.submitted2015-02-09
dc.identifier.citationHum Gene Ther. 2013 Sep;24(9):814-9. doi: 10.1089/hum.2013.118. <a href="http://dx.doi.org/10.1089/hum.2013.118">Link to article on publisher's website</a>
dc.identifier.issn1557-7422
dc.identifier.doi10.1089/hum.2013.118
dc.identifier.pmid24010702
dc.identifier.urihttp://hdl.handle.net/20.500.14038/30291
dc.description.abstractA 66-year-old woman heterozygous for a mutation in the ornithine transcarbamylase gene (Otc) participated in a phase I gene therapy trial for OTC deficiency. She received an adenovirus (Ad) vector expressing the functional OTC gene by intraportal perfusion. Fourteen years later she developed and subsequently died of hepatocellular carcinoma. A second subject, a 45-year-old woman, enrolled in the same trial presented with colon cancer 15 years later. We sought to investigate a possible association between the development of a tumor and prior adenoviral gene transfer in these two subjects. We developed and validated a sensitive nested polymerase chain reaction assay for recovering recombinant Ad sequences from host tissues. Using this method, we could not detect any Ad vector DNA in either tumor or normal tissue from the two patients. Our results are informative in ruling out the possibility that the adenoviral vector might have contributed to the development of cancer in those two subjects.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=24010702&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768231/
dc.subjectAged
dc.subjectCarcinoma, Hepatocellular
dc.subjectColorectal Neoplasms
dc.subjectDependovirus
dc.subjectFemale
dc.subjectGene Transfer Techniques
dc.subjectGenetic Therapy
dc.subjectHumans
dc.subjectLiver Neoplasms
dc.subjectMiddle Aged
dc.subjectOrnithine Carbamoyltransferase
dc.subjectOrnithine Carbamoyltransferase Deficiency Disease
dc.subjectResearch Subjects
dc.subjectCongenital, Hereditary, and Neonatal Diseases and Abnormalities
dc.subjectGenetic Processes
dc.subjectGenetics
dc.subjectMolecular Genetics
dc.subjectNeoplasms
dc.subjectNervous System Diseases
dc.subjectTherapeutics
dc.titleVector sequences are not detected in tumor tissue from research subjects with ornithine transcarbamylase deficiency who previously received adenovirus gene transfer
dc.typeJournal Article
dc.source.journaltitleHuman gene therapy
dc.source.volume24
dc.source.issue9
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/faculty_pubs/543
dc.identifier.contextkey6627824
html.description.abstract<p>A 66-year-old woman heterozygous for a mutation in the ornithine transcarbamylase gene (Otc) participated in a phase I gene therapy trial for OTC deficiency. She received an adenovirus (Ad) vector expressing the functional OTC gene by intraportal perfusion. Fourteen years later she developed and subsequently died of hepatocellular carcinoma. A second subject, a 45-year-old woman, enrolled in the same trial presented with colon cancer 15 years later. We sought to investigate a possible association between the development of a tumor and prior adenoviral gene transfer in these two subjects. We developed and validated a sensitive nested polymerase chain reaction assay for recovering recombinant Ad sequences from host tissues. Using this method, we could not detect any Ad vector DNA in either tumor or normal tissue from the two patients. Our results are informative in ruling out the possibility that the adenoviral vector might have contributed to the development of cancer in those two subjects.</p>
dc.identifier.submissionpathfaculty_pubs/543
dc.contributor.departmentDepartment of Microbiology and Physiology Systems
dc.contributor.departmentDivision of Hematology/Oncology, Department of Pediatrics
dc.contributor.departmentGene Therapy Center
dc.source.pages814-9


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