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dc.contributor.authorde Vrij, Jeroen
dc.contributor.authorMaas, Sybren L.N.
dc.contributor.authorvan Nispen, Malisa
dc.contributor.authorSena-Esteves, Miguel
dc.contributor.authorLimpens, Ronald W.A.
dc.contributor.authorKoster, Abraham J.
dc.contributor.authorLeenstra, Sieger
dc.contributor.authorLamfers, Martine L.
dc.contributor.authorBroekman, Marike L. D
dc.date2022-08-11T08:08:31.000
dc.date.accessioned2022-08-23T15:57:54Z
dc.date.available2022-08-23T15:57:54Z
dc.date.issued2013-09-01
dc.date.submitted2015-03-24
dc.identifier.citation<p>Nanomedicine (Lond). 2013 Sep;8(9):1443-58. doi: 10.2217/nnm.12.173. Epub 2013 Feb 5. <a href="http://dx.doi.org/10.2217/nnm.12.173">Link to article on publisher's site</a></p>
dc.identifier.issn1743-5889 (Linking)
dc.identifier.doi10.2217/nnm.12.173
dc.identifier.pmid23384702
dc.identifier.urihttp://hdl.handle.net/20.500.14038/30308
dc.description.abstractBACKGROUND: Cells secrete different types of membrane vesicles (MVs), which may act as important entities in normal human physiology and in various pathological processes. The established methods for quantification of MVs require purification or preanalytical handling of samples with labeling moieties. AIM: The authors' aim was to develop a method for high-throughput, labeling-free quantification of nonpurified MVs. MATERIALS and METHODS: Scanning ion occlusion sensing technology, which relies on the detection of particles upon their movement through a nanopore, was investigated for the ability to quantify nanosized MVs ( < 400 nm) in bodily fluids and cell culture supernatants. RESULTS: Scanning ion occlusion sensing allowed for rapid and easy measurement of the concentration of MVs in all biological fluids tested. CONCLUSION: Scanning ion occlusion sensing technology enables the quantification of MVs in biological samples without the requirement of MV isolation and/or labeling. This offers a highly valuable addition to the currently used repertoire of MV quantification methods.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=23384702&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.2217/nnm.12.173
dc.subjectBiological Transport
dc.subjectBody Fluids
dc.subjectHumans
dc.subjectIons
dc.subjectMembranes
dc.subject*Nanopores
dc.subjectParticle Size
dc.subjectSecretory Vesicles
dc.subjectNanomedicine
dc.titleQuantification of nanosized extracellular membrane vesicles with scanning ion occlusion sensing
dc.typeJournal Article
dc.source.journaltitleNanomedicine (London, England)
dc.source.volume8
dc.source.issue9
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/faculty_pubs/569
dc.identifier.contextkey6889232
html.description.abstract<p>BACKGROUND: Cells secrete different types of membrane vesicles (MVs), which may act as important entities in normal human physiology and in various pathological processes. The established methods for quantification of MVs require purification or preanalytical handling of samples with labeling moieties. AIM: The authors' aim was to develop a method for high-throughput, labeling-free quantification of nonpurified MVs.</p> <p>MATERIALS and METHODS: Scanning ion occlusion sensing technology, which relies on the detection of particles upon their movement through a nanopore, was investigated for the ability to quantify nanosized MVs ( < 400 nm) in bodily fluids and cell culture supernatants.</p> <p>RESULTS: Scanning ion occlusion sensing allowed for rapid and easy measurement of the concentration of MVs in all biological fluids tested.</p> <p>CONCLUSION: Scanning ion occlusion sensing technology enables the quantification of MVs in biological samples without the requirement of MV isolation and/or labeling. This offers a highly valuable addition to the currently used repertoire of MV quantification methods.</p>
dc.identifier.submissionpathfaculty_pubs/569
dc.contributor.departmentDepartment of Neurology, Gene Therapy Center
dc.source.pages1443-58


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