Expression of inhibitory markers is increased on effector memory T cells during hepatitis C virus/HIV coinfection as compared to hepatitis C virus or HIV monoinfection
UMass Chan Affiliations
Department of Medicine, Division of GastroenterologyDocument Type
Journal ArticlePublication Date
2013-09-10Keywords
AdultAntigens, CD
Cell Proliferation
Cells, Cultured
Coinfection
Cytokines
HIV Infections
Hepatitis C
Humans
Immunologic Memory
Immunophenotyping
Leukocytes, Mononuclear
Male
Membrane Proteins
Middle Aged
Programmed Cell Death 1 Receptor
T-Lymphocyte Subsets
Young Adult
Gastroenterology
Hepatology
Immunopathology
Virus Diseases
Metadata
Show full item recordAbstract
OBJECTIVE: Hepatitis C virus (HCV)/HIV coinfection is associated with rapid progression of hepatic fibrosis and liver disease. T-cell response has been implicated in the pathophysiological outcome of the disease. DESIGN: This study sought to evaluate the role of memory T-cell exhaustion in enhancing immune dysfunction during coinfection. METHODS: Sixty-four patients were included in the study; HCV monoinfected (n = 21), HIV monoinfected (n = 23), HCV/HIV coinfected (n = 20), and healthy controls (n = 20). Peripheral blood mononuclear cells (PBMCs) were isolated; immunophenotyped and functional assays were performed. RESULTS: A significant increase in the naive T cells and central memory T cells and a marked reduction in effector memory T cells (TEM) were observed with coinfection as compared to monoinfection. Inhibitory markers programmed death 1 (PD-1) and T-cell immunoglobulin and mucin domain containing molecule 3 (TIM3) were highly upregulated on TEM in coinfection and functionally, these TEM cells displayed lowered proliferation. Increased expression of PD-1 and TIM3 correlated with decreased levels of CD8+CD107a+ TEM cells in coinfection. Pro-inflammatory cytokines interferon-gamma and interleukin-2 (IL-2) secretion by TEM cells were also reduced during chronic viral infection. Secretion of IL-10, a human cytokine synthesis inhibitory factor, was significantly upregulated in CD4 TEM with HCV/HIV coinfection in comparison to HCV monoinfection. CONCLUSION: TEM cells play an important role during viral infection and enhanced expression of inhibitory markers is associated with decreased proliferation and cytotoxicity and increased IL-10 production, which was pronounced in HCV/HIV coinfection. Thus, decreased TEM functionality contributes to diminished host immune responses during HCV/HIV coinfection as compared to HCV or HIV monoinfection.Source
AIDS. 2013 Sep 10;27(14):2191-200. doi: 10.1097/QAD.0b013e32836285e4. Link to article on publisher's siteDOI
10.1097/QAD.0b013e32836285e4Permanent Link to this Item
http://hdl.handle.net/20.500.14038/30333PubMed ID
23820090Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1097/QAD.0b013e32836285e4