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dc.contributor.authorSage, Jay M.
dc.contributor.authorKnight, Kendall L.
dc.date2022-08-11T08:08:34.000
dc.date.accessioned2022-08-23T15:59:27Z
dc.date.available2022-08-23T15:59:27Z
dc.date.issued2013-07-01
dc.date.submitted2013-07-02
dc.identifier.citationMitochondrion. 2013 Jul;13(4):350-6. doi: 10.1016/j.mito.2013.04.004. <a href="http://dx.doi.org/10.1016/j.mito.2013.04.004">Link to article on publisher's site</a>
dc.identifier.issn1567-7249 (Linking)
dc.identifier.doi10.1016/j.mito.2013.04.004
dc.identifier.pmid23591384
dc.identifier.urihttp://hdl.handle.net/20.500.14038/30671
dc.description.abstractHomologous recombination is essential for productive DNA replication particularly under stress conditions. We previously demonstrated a stress-induced recruitment of Rad51 to mitochondria and a critical need for its activity in the maintenance of mitochondrial DNA (mtDNA) copy number. Using the human osteosarcoma cell line U20S, we show in the present study that recruitment of Rad51 to mitochondria under stress conditions requires ongoing mtDNA replication. Additionally, Rad51 levels in mitochondria increase in cells recovering from mtDNA depletion. Our findings highlight an important new role for Rad51 in supporting mtDNA replication, and further promote the idea that recombination is indispensable for sustaining DNA synthesis under conditions of replication stress.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=23591384&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/j.mito.2013.04.004
dc.subjectRad51 Recombinase
dc.subjectDNA, Mitochondrial
dc.subjectDNA Replication
dc.subjectBiochemistry
dc.subjectMolecular Biology
dc.subjectMolecular Genetics
dc.titleHuman Rad51 promotes mitochondrial DNA synthesis under conditions of increased replication stress
dc.typeJournal Article
dc.source.journaltitleMitochondrion
dc.source.volume13
dc.source.issue4
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/faculty_pubs/95
dc.identifier.contextkey4276310
html.description.abstract<p>Homologous recombination is essential for productive DNA replication particularly under stress conditions. We previously demonstrated a stress-induced recruitment of Rad51 to mitochondria and a critical need for its activity in the maintenance of mitochondrial DNA (mtDNA) copy number. Using the human osteosarcoma cell line U20S, we show in the present study that recruitment of Rad51 to mitochondria under stress conditions requires ongoing mtDNA replication. Additionally, Rad51 levels in mitochondria increase in cells recovering from mtDNA depletion. Our findings highlight an important new role for Rad51 in supporting mtDNA replication, and further promote the idea that recombination is indispensable for sustaining DNA synthesis under conditions of replication stress.</p>
dc.identifier.submissionpathfaculty_pubs/95
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.source.pages350-6


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