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dc.contributor.authorChou, Hsin-Jung
dc.contributor.authorDonnard, Elisa
dc.contributor.authorGustafsson, H. Tobias
dc.contributor.authorGarber, Manuel
dc.contributor.authorRando, Oliver J.
dc.date2022-08-11T08:08:36.000
dc.date.accessioned2022-08-23T16:01:05Z
dc.date.available2022-08-23T16:01:05Z
dc.date.issued2017-12-07
dc.date.submitted2018-07-18
dc.identifier.citation<p>Mol Cell. 2017 Dec 7;68(5):978-992.e4. doi: 10.1016/j.molcel.2017.11.002. Epub 2017 Nov 30. <a href="https://doi.org/10.1016/j.molcel.2017.11.002">Link to article on publisher's site</a></p>
dc.identifier.issn1097-2765 (Linking)
dc.identifier.doi10.1016/j.molcel.2017.11.002
dc.identifier.pmid29198561
dc.identifier.urihttp://hdl.handle.net/20.500.14038/31039
dc.description.abstractCovalent nucleotide modifications in noncoding RNAs affect a plethora of biological processes, and new functions continue to be discovered even for well-known modifying enzymes. To systematically compare the functions of a large set of noncoding RNA modifications in gene regulation, we carried out ribosome profiling in budding yeast to characterize 57 nonessential genes involved in tRNA modification. Deletion mutants exhibited a range of translational phenotypes, with enzymes known to modify anticodons, or non-tRNA substrates such as rRNA, exhibiting the most dramatic translational perturbations. Our data build on prior reports documenting translational upregulation of the nutrient-responsive transcription factor Gcn4 in response to numerous tRNA perturbations, and identify many additional translationally regulated mRNAs throughout the yeast genome. Our data also uncover unexpected roles for tRNA-modifying enzymes in regulation of TY retroelements, and in rRNA 2'-O-methylation. This dataset should provide a rich resource for discovery of additional links between tRNA modifications and gene regulation.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=29198561&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1016/j.molcel.2017.11.002
dc.subjectRNA modifications
dc.subjectnoncoding RNA
dc.subjectprotein translation
dc.subjectribosome profiling
dc.subjecttRNA
dc.subjectAmino Acids, Peptides, and Proteins
dc.subjectBioinformatics
dc.subjectComputational Biology
dc.subjectEnzymes and Coenzymes
dc.subjectGenetic Phenomena
dc.subjectGenomics
dc.subjectIntegrative Biology
dc.subjectMolecular Biology
dc.subjectNucleic Acids, Nucleotides, and Nucleosides
dc.titleTranscriptome-wide Analysis of Roles for tRNA Modifications in Translational Regulation
dc.typeArticle
dc.source.journaltitleMolecular cell
dc.source.volume68
dc.source.issue5
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/garber_lab_pubs/7
dc.identifier.contextkey12504421
html.description.abstract<p>Covalent nucleotide modifications in noncoding RNAs affect a plethora of biological processes, and new functions continue to be discovered even for well-known modifying enzymes. To systematically compare the functions of a large set of noncoding RNA modifications in gene regulation, we carried out ribosome profiling in budding yeast to characterize 57 nonessential genes involved in tRNA modification. Deletion mutants exhibited a range of translational phenotypes, with enzymes known to modify anticodons, or non-tRNA substrates such as rRNA, exhibiting the most dramatic translational perturbations. Our data build on prior reports documenting translational upregulation of the nutrient-responsive transcription factor Gcn4 in response to numerous tRNA perturbations, and identify many additional translationally regulated mRNAs throughout the yeast genome. Our data also uncover unexpected roles for tRNA-modifying enzymes in regulation of TY retroelements, and in rRNA 2'-O-methylation. This dataset should provide a rich resource for discovery of additional links between tRNA modifications and gene regulation.</p>
dc.identifier.submissionpathgarber_lab_pubs/7
dc.contributor.departmentGarber Lab
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentProgram in Bioinformatics and Integrative Biology
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.source.pages978-992.e4


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