Semiconductor-based DNA sequencing of histone modification states
Histone post-translational modifications
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AbstractThe recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues.
Nat Commun. 2013;4:2672. doi: 10.1038/ncomms3672. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/31040
Full author list omitted for brevity. For the full list of authors, see article.