Semiconductor-based DNA sequencing of histone modification states
| dc.contributor.author | Cheng, Christine S. | |
| dc.contributor.author | Garber, Manuel | |
| dc.contributor.author | Chin, Lynda | |
| dc.contributor.author | Regev, Aviv | |
| dc.contributor.author | Amit, Ido | |
| dc.date | 2022-08-11T08:08:36.000 | |
| dc.date.accessioned | 2022-08-23T16:01:05Z | |
| dc.date.available | 2022-08-23T16:01:05Z | |
| dc.date.issued | 2013-10-25 | |
| dc.date.submitted | 2018-07-18 | |
| dc.identifier.citation | <p>Nat Commun. 2013;4:2672. doi: 10.1038/ncomms3672. <a href="https://doi.org/10.1038/ncomms3672">Link to article on publisher's site</a></p> | |
| dc.identifier.issn | 2041-1723 (Linking) | |
| dc.identifier.doi | 10.1038/ncomms3672 | |
| dc.identifier.pmid | 24157732 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14038/31040 | |
| dc.description | <p>Full author list omitted for brevity. For the full list of authors, see article.</p> | |
| dc.description.abstract | The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues. | |
| dc.language.iso | en_US | |
| dc.relation | <p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=24157732&dopt=Abstract">Link to Article in PubMed</a></p> | |
| dc.relation.url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917140/ | |
| dc.subject | Cancer | |
| dc.subject | DNA sequencing | |
| dc.subject | Epigenetics | |
| dc.subject | Histone post-translational modifications | |
| dc.subject | Bioinformatics | |
| dc.subject | Computational Biology | |
| dc.subject | Genomics | |
| dc.subject | Molecular Biology | |
| dc.title | Semiconductor-based DNA sequencing of histone modification states | |
| dc.type | Journal Article | |
| dc.source.journaltitle | Nature communications | |
| dc.source.volume | 4 | |
| dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/garber_lab_pubs/8 | |
| dc.identifier.contextkey | 12504423 | |
| html.description.abstract | <p>The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues.</p> | |
| dc.identifier.submissionpath | garber_lab_pubs/8 | |
| dc.contributor.department | Garber Lab | |
| dc.contributor.department | Program in Bioinformatics and Integrative Biology | |
| dc.source.pages | 2672 |