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dc.contributor.advisorPhillip D. Zamore, Ph.D.
dc.contributor.authorMatranga, Christian B.
dc.date2022-08-11T08:08:41.000
dc.date.accessioned2022-08-23T16:04:19Z
dc.date.available2022-08-23T16:04:19Z
dc.date.issued2007-09-17
dc.date.submitted2008-01-25
dc.identifier.doi10.13028/amen-ke39
dc.identifier.urihttp://hdl.handle.net/20.500.14038/31665
dc.description.abstractIn 1990, Richard Jorgensen’s lab initiated a study to test if they could create a more vivid color petunia (Napoli et al. 1990). Their plan was to transform plants with the chalcone synthase transgene––the predicted rate limiting factor in the production of purple pigmentation. Much to their surprise, the transgenic plants, as well as their progeny, displayed a great reduction in pigmentation. This loss of endogenous function was termed “cosuppression” and it was thought that sequence-specific repression resulted from over-expression of the homologous transgene sequence. In 1998, Andrew Fire and Craig Mello described a phenomenon in which double stranded RNA (dsRNA) can trigger silencing of cognate sequences when injected into the nematode, Caenorhabditis elegans (Fire et al. 1998). This data explained observations seen years earlier by other worm researchers, and suggested that repression of pigmentation in plants was caused by a dsRNA-intermediate (Guo and Kemphues 1995; Napoli et al. 1990). The phenomenon––which soon after was coined RNA interference (RNAi)––was soon discovered to be a post-transcriptional surveillance system in plants and animals to remove foreign nucleic acids.
dc.language.isoen_US
dc.rightsCopyright is held by the author, with all rights reserved.
dc.subjectRNA Interference
dc.subjectMicroRNAs
dc.subjectRNA
dc.subjectSmall Interfering
dc.subjectDrosophila Proteins
dc.subjectMethyltransferases
dc.subjectRNA-Induced Silencing Complex
dc.subjectRNA 3' End Processing
dc.subjectPlants
dc.subjectRNA Helicases
dc.subjectRNA-Binding Proteins
dc.subjectAmino Acids, Peptides, and Proteins
dc.subjectAnimal Experimentation and Research
dc.subjectEnzymes and Coenzymes
dc.subjectNucleic Acids, Nucleotides, and Nucleosides
dc.titleUnderstanding Assembly of AGO2 RISC: the RNAi enzyme: a Dissertation
dc.typeDoctoral Dissertation
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1347&context=gsbs_diss&unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_diss/347
dc.legacy.embargo2017-04-24T00:00:00-07:00
dc.identifier.contextkey418400
refterms.dateFOA2022-08-27T04:44:37Z
html.description.abstract<p>In 1990, Richard Jorgensen’s lab initiated a study to test if they could create a more vivid color petunia (Napoli et al. 1990). Their plan was to transform plants with the chalcone synthase transgene––the predicted rate limiting factor in the production of purple pigmentation. Much to their surprise, the transgenic plants, as well as their progeny, displayed a great reduction in pigmentation. This loss of endogenous function was termed “cosuppression” and it was thought that sequence-specific repression resulted from over-expression of the homologous transgene sequence. In 1998, Andrew Fire and Craig Mello described a phenomenon in which double stranded RNA (dsRNA) can trigger silencing of cognate sequences when injected into the nematode, Caenorhabditis elegans (Fire et al. 1998). This data explained observations seen years earlier by other worm researchers, and suggested that repression of pigmentation in plants was caused by a dsRNA-intermediate (Guo and Kemphues 1995; Napoli et al. 1990). The phenomenon––which soon after was coined RNA interference (RNAi)––was soon discovered to be a post-transcriptional surveillance system in plants and animals to remove foreign nucleic acids.</p>
dc.identifier.submissionpathgsbs_diss/347
dc.contributor.departmentRNA Therapeutics Institute
dc.description.thesisprogramBiochemistry and Molecular Pharmacology


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