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    Roles for Histones H4 Serine 1 Phosphorylation in DNA Double Strand Break Repair and Chromatin Compaction: A Dissertation

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    Authors
    Foley, Melissa Anne
    Faculty Advisor
    Craig Peterson, Ph.D.
    Academic Program
    Interdisciplinary Graduate Program
    UMass Chan Affiliations
    Program in Molecular Medicine
    Document Type
    Doctoral Dissertation
    Publication Date
    2008-08-14
    Keywords
    DNA Repair
    DNA Damage
    Casein Kinase II
    Histones
    Saccharomyces cerevisiae
    Saccharomyces cerevisiae Proteins
    Amino Acids, Peptides, and Proteins
    Cells
    Enzymes and Coenzymes
    Fungi
    Genetic Phenomena
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    Abstract
    The study of DNA templated events is not complete without considering the chromatin environment. Histone modifications help to regulate gene expression, chromatin compaction and DNA replication. Because DNA damage repair must occur within the context of chromatin, many remodeling enzymes and histone modifications work in concert to enable access to the DNA and aid in restoration of chromatin after repair is complete. CK2 has recently been identified as a histone modifying enzyme. In this study we identify CK2 as a histone H3 tail kinase in vitro, identify the phospho-acceptor site in vitro, and characterize the modification in vivo in S. cerevisiae. We also characterize the DNA damage phenotype of a strain lacking a single catalytic subunit of CK2. We further characterize the CK2- dependent phosphorylation of serine 1 of histone H4 in vivo. We find that it is recruited directly to the site of a DSB and this recruitment requires the SIN3/RPD3 histone deacetylase complex. We also characterize the contribution of H4 serine 1 phosphorylation in chromatin compaction by using reconstituted nucleosomal arrays to study folding in the analytical ultracentrifuge.
    DOI
    10.13028/shgk-q661
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/31709
    Rights
    Copyright is held by the author, with all rights reserved.
    ae974a485f413a2113503eed53cd6c53
    10.13028/shgk-q661
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