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    Serotonin-Expressing Cells in the Corpus of the Stomach Originate from Bone Marrow: A Master’s Thesis

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    Authors
    Johnston, Brian T.
    Faculty Advisor
    JeanMarie Houghton, M.D., Ph.D.
    Academic Program
    Interdisciplinary Graduate Program
    UMass Chan Affiliations
    Medicine
    Document Type
    Master's Thesis
    Publication Date
    2012-08-27
    Keywords
    Stomach
    Serotonin
    Bone Marrow Cells
    Nerve Tissue Proteins
    Basic Helix-Loop-Helix Transcription Factors
    Amino Acids, Peptides, and Proteins
    Animal Experimentation and Research
    Biological Factors
    Cell and Developmental Biology
    Cells
    Digestive System
    Hemic and Immune Systems
    Heterocyclic Compounds
    Hormones, Hormone Substitutes, and Hormone Antagonists
    Organic Chemicals
    Tissues
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    Abstract
    Neurogenin 3 and its downstream target NeuroD are basic helix-loop-helix transcription factors which promote endocrine differentiation in the gastrointestinal tract. However, mice lacking Ngn3 still produce several hormones in the stomach. Lineage tracing mouse models demonstrated that a majority of hormone cells in the corpus region of the stomach did not express Ngn3 or NeuroD during differentiation. Serotonin and histamine cells were entirely NeuroD-independently derived, and serotonin cells were additionally entirely Ngn3-independently derived. In this study, we isolated serotonin and histamine cells from the gastric corpus of transgenic mice expressing the fluorescent marker CFP. Serotonin cells expressed multiple mast cell markers by RT-PCR, and were found to be nearly absent in a mast cell-deficient mouse model. Labeled bone marrow transplant mice showed all serotonin cells derived from bone marrow. Histamine-expressing ECL cells, while lacking NeuroD, did not appear to express granulocyte or mast cell markers by analytical flow cytometry and RT-PCR, and resemble other enteroendocrine cell populations. Mouse gastric corpus serotonin cells, but not antral serotonin cells, are bone marrow-derived mast cells.
    DOI
    10.13028/e47j-b396
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/31989
    Rights
    Copyright is held by the author, with all rights reserved.
    ae974a485f413a2113503eed53cd6c53
    10.13028/e47j-b396
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    Morningside Graduate School of Biomedical Sciences Dissertations and Theses

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