Morphogenetic Requirements for Embryo Patterning and the Generation of Stem Cell-derived Mice: A Dissertation
Authors
Yoon, YeonsooFaculty Advisor
Jaime A. Rivera-Perez, PhDAcademic Program
Cell BiologyUMass Chan Affiliations
PediatricsDocument Type
Doctoral DissertationPublication Date
2013-07-15Keywords
Dissertations, UMMSAurora Kinase A
Body Patterning
Cell Proliferation
Embryo, Mammalian
Embryonic Stem Cells
Aurora Kinase A
Body Patterning
Cell Proliferation
Mammalian Embryo
Embryonic Stem Cells
Cell Biology
Cellular and Molecular Physiology
Developmental Biology
Metadata
Show full item recordAbstract
Cell proliferation and differentiation are tightly regulated processes required for the proper development of multi-cellular organisms. To understand the effects of cell proliferation on embryo patterning in mice, we inactivated Aurora A, a gene essential for completion of the cell cycle. We discovered that inhibiting cell proliferation leads to different outcomes depending on the tissue affected. If the epiblast, the embryonic component, is compromised, it leads to gastrulation failure. However, when Aurora A is inactivated in extra-embryonic tissues, mutant embryos fail to properly establish the anteroposterior axis. Ablation of Aurora A in the epiblast eventually leads to abnormal embryos composed solely of extra-embryonic tissues. We took advantage of this phenomenon to generate embryonic stem (ES) cell-derived mice. We successfully generated newborn pups using this epiblast ablation chimera strategy. Our results highlight the importance of coordinated cell proliferation events in embryo patterning. In addition, epiblast ablation chimeras provide a novel in vivo assay for pluripotency that is simpler and more amenable to use by stem cell researchers.DOI
10.13028/M2ZS3WPermanent Link to this Item
http://hdl.handle.net/20.500.14038/32033Rights
Copyright is held by the author, with all rights reserved.ae974a485f413a2113503eed53cd6c53
10.13028/M2ZS3W