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dc.contributor.advisorJaime A. Rivera-Perez, PhD
dc.contributor.authorYoon, Yeonsoo
dc.date2022-08-11T08:08:44.000
dc.date.accessioned2022-08-23T16:06:18Z
dc.date.available2022-08-23T16:06:18Z
dc.date.issued2013-07-15
dc.date.submitted2013-12-23
dc.identifier.doi10.13028/M2ZS3W
dc.identifier.urihttp://hdl.handle.net/20.500.14038/32033
dc.description.abstractCell proliferation and differentiation are tightly regulated processes required for the proper development of multi-cellular organisms. To understand the effects of cell proliferation on embryo patterning in mice, we inactivated Aurora A, a gene essential for completion of the cell cycle. We discovered that inhibiting cell proliferation leads to different outcomes depending on the tissue affected. If the epiblast, the embryonic component, is compromised, it leads to gastrulation failure. However, when Aurora A is inactivated in extra-embryonic tissues, mutant embryos fail to properly establish the anteroposterior axis. Ablation of Aurora A in the epiblast eventually leads to abnormal embryos composed solely of extra-embryonic tissues. We took advantage of this phenomenon to generate embryonic stem (ES) cell-derived mice. We successfully generated newborn pups using this epiblast ablation chimera strategy. Our results highlight the importance of coordinated cell proliferation events in embryo patterning. In addition, epiblast ablation chimeras provide a novel in vivo assay for pluripotency that is simpler and more amenable to use by stem cell researchers.
dc.language.isoen_US
dc.rightsCopyright is held by the author, with all rights reserved.
dc.subjectDissertations, UMMS
dc.subjectAurora Kinase A
dc.subjectBody Patterning
dc.subjectCell Proliferation
dc.subjectEmbryo, Mammalian
dc.subjectEmbryonic Stem Cells
dc.subjectAurora Kinase A
dc.subjectBody Patterning
dc.subjectCell Proliferation
dc.subjectMammalian Embryo
dc.subjectEmbryonic Stem Cells
dc.subjectCell Biology
dc.subjectCellular and Molecular Physiology
dc.subjectDevelopmental Biology
dc.titleMorphogenetic Requirements for Embryo Patterning and the Generation of Stem Cell-derived Mice: A Dissertation
dc.typeDoctoral Dissertation
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1682&context=gsbs_diss&unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_diss/679
dc.legacy.embargo2014-08-29T00:00:00-07:00
dc.identifier.contextkey4943399
refterms.dateFOA2022-08-26T04:09:28Z
html.description.abstract<p>Cell proliferation and differentiation are tightly regulated processes required for the proper development of multi-cellular organisms. To understand the effects of cell proliferation on embryo patterning in mice, we inactivated <em>Aurora A</em>, a gene essential for completion of the cell cycle. We discovered that inhibiting cell proliferation leads to different outcomes depending on the tissue affected. If the epiblast, the embryonic component, is compromised, it leads to gastrulation failure. However, when <em>Aurora A</em> is inactivated in extra-embryonic tissues, mutant embryos fail to properly establish the anteroposterior axis. Ablation of<em> Aurora A</em> in the epiblast eventually leads to abnormal embryos composed solely of extra-embryonic tissues. We took advantage of this phenomenon to generate embryonic stem (ES) cell-derived mice. We successfully generated newborn pups using this epiblast ablation chimera strategy. Our results highlight the importance of coordinated cell proliferation events in embryo patterning. In addition, epiblast ablation chimeras provide a novel <em>in vivo</em> assay for pluripotency that is simpler and more amenable to use by stem cell researchers.</p>
dc.identifier.submissionpathgsbs_diss/679
dc.contributor.departmentPediatrics
dc.description.thesisprogramCell Biology


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