Characterization of the Visceral Endoderm Components in Early Post-Implantation Mouse Embryo Development: A Dissertation
Faculty AdvisorJaime Rivera Perez, PhD
Academic ProgramCell Biology
UMass Chan AffiliationsPediatrics
Document TypeDoctoral Dissertation
Cellular and Molecular Physiology
MetadataShow full item record
AbstractEarly post-implantation vertebrate embryos are shaped by complex cellular and molecular mechanisms. In mice, the visceral endoderm, an extraembryonic cell lineage that appears before gastrulation, provides several important functions such as nutrition and mechanical protection. My thesis research focused on the role of the visceral endoderm in embryo patterning, a newly discovered function for this tissue. My results showed that an interplay between two subpopulations of visceral endoderm the anterior and posterior visceral endoderm, located on the opposite sides of the developing conceptus, are critical for the establishment of the anteroposterior body axis of the embryo. I also found that senescence-associated β-galactosidase activity delineates the visceral endoderm marking apical vacuole, a lysosomal-like organelle. This however indicates the nutritional function of visceral endoderm cells rather than a senescent population. My studies highlight the fundamental role of extraembryonic tissues in patterning mammalian embryos as opposed to housekeeping roles. They also reveal important difference when conducting studies at the organismal level rather than in cells in culture.
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/32050
RightsCopyright is held by the author, with all rights reserved.