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dc.contributor.authorLiu, Zhaohui M.
dc.contributor.authorSchmidt, Karl F.
dc.contributor.authorSicard, Kenneth M.
dc.contributor.authorDuong, Timothy Q.
dc.date2022-08-11T08:08:47.000
dc.date.accessioned2022-08-23T16:08:20Z
dc.date.available2022-08-23T16:08:20Z
dc.date.issued2004-07-30
dc.date.submitted2009-09-14
dc.identifier.issn0740-3194 (Print)
dc.identifier.pmid15282809
dc.identifier.urihttp://hdl.handle.net/20.500.14038/32421
dc.description<p>Co-author <a>Karl F. Schmidt</a> is a student in the Neuroscience program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.</p>
dc.description.abstractThe cerebral metabolic rate of oxygen (CMRO2) was dynamically evaluated on a pixel-by-pixel basis in isoflurane-anesthetized and spontaneously breathing rats following graded electrical somatosensory forepaw stimulations (4, 6, and 8 mA). In contrast to alpha-chloralose, which is the most widely used anesthetic in forepaw-stimulation fMRI studies of rats under mechanical ventilation, isoflurane (1.1-1.2%) provided a stable anesthesia level over a prolonged period, without the need to adjust the ventilation volume/rate or sample blood gases. Combined cerebral blood flow signals (CBF) and blood oxygenation level-dependent (BOLD) fMRI signals were simultaneously measured with the use of a multislice continuous arterial spin labeling (CASL) technique (two-coil setup). CMRO2 was calculated using the biophysical BOLD model of Ogawa et al. (Proc Natl Acad Sci USA 1992;89:5951-5955). The stimulus-evoked BOLD percent changes at 4, 6, and 8 A were, respectively, 0.5% +/- 0.2%, 1.4% +/- 0.3%, and 2.0% +/- 0.3% (mean +/- SD, N = 6). The CBF percent changes were 23% +/- 6%, 58% +/- 9%, and 87% +/- 14%. The CMRO2 percent changes were 14% +/- 4%, 24% +/- 6%, and 43% +/- 11%. BOLD, CBF, and CMRO2 activations were localized to the forepaw somatosensory cortices without evidence of plateau for oxygen consumption, indicative of partial coupling of CBF and CMRO2. This study describes a useful forepaw-stimulation model for fMRI, and demonstrate that CMRO2 changes can be dynamically imaged on a pixel-by-pixel basis in a single setting with high spatiotemporal resolution.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=15282809&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1002/mrm.20148
dc.rightsCitation: Magn Reson Med. 2004 Aug;52(2):277-85. <a href="http://dx.doi.org/10.1002/mrm.20148">Link to article on publisher's site</a>
dc.subjectAnesthetics, Inhalation
dc.subjectAnimals
dc.subjectBrain
dc.subjectBrain Mapping
dc.subjectCerebrovascular Circulation
dc.subjectElectric Stimulation
dc.subjectForelimb
dc.subjectIsoflurane
dc.subjectMagnetic Resonance Imaging
dc.subjectMale
dc.subjectOxygen
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectRespiration
dc.subjectRespiration, Artificial
dc.subjectSpin Labels
dc.subjectNeuroscience and Neurobiology
dc.titleImaging oxygen consumption in forepaw somatosensory stimulation in rats under isoflurane anesthesia
dc.typeJournal Article
dc.source.journaltitleMagnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine
dc.source.volume52
dc.source.issue2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_mdphd/4
dc.identifier.contextkey1004103
html.description.abstract<p>The cerebral metabolic rate of oxygen (CMRO2) was dynamically evaluated on a pixel-by-pixel basis in isoflurane-anesthetized and spontaneously breathing rats following graded electrical somatosensory forepaw stimulations (4, 6, and 8 mA). In contrast to alpha-chloralose, which is the most widely used anesthetic in forepaw-stimulation fMRI studies of rats under mechanical ventilation, isoflurane (1.1-1.2%) provided a stable anesthesia level over a prolonged period, without the need to adjust the ventilation volume/rate or sample blood gases. Combined cerebral blood flow signals (CBF) and blood oxygenation level-dependent (BOLD) fMRI signals were simultaneously measured with the use of a multislice continuous arterial spin labeling (CASL) technique (two-coil setup). CMRO2 was calculated using the biophysical BOLD model of Ogawa et al. (Proc Natl Acad Sci USA 1992;89:5951-5955). The stimulus-evoked BOLD percent changes at 4, 6, and 8 A were, respectively, 0.5% +/- 0.2%, 1.4% +/- 0.3%, and 2.0% +/- 0.3% (mean +/- SD, N = 6). The CBF percent changes were 23% +/- 6%, 58% +/- 9%, and 87% +/- 14%. The CMRO2 percent changes were 14% +/- 4%, 24% +/- 6%, and 43% +/- 11%. BOLD, CBF, and CMRO2 activations were localized to the forepaw somatosensory cortices without evidence of plateau for oxygen consumption, indicative of partial coupling of CBF and CMRO2. This study describes a useful forepaw-stimulation model for fMRI, and demonstrate that CMRO2 changes can be dynamically imaged on a pixel-by-pixel basis in a single setting with high spatiotemporal resolution.</p>
dc.identifier.submissionpathgsbs_mdphd/4
dc.contributor.departmentGraduate School of Biomedical Sciences, MD/PhD Program
dc.contributor.departmentDepartment of Psychiatry, Center for Comparative NeuroImaging
dc.source.pages277-85
dc.contributor.studentKarl F. Schmidt; Kenneth Sicard


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