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dc.contributor.authorHenninger, Nils
dc.contributor.authorEberius, Karl H.
dc.contributor.authorSicard, Kenneth M.
dc.contributor.authorKollmar, Rainer
dc.contributor.authorSommer, Clemens
dc.contributor.authorSchwab, Stefan
dc.contributor.authorSchabitz, Wolf-Rudiger
dc.date2022-08-11T08:08:47.000
dc.date.accessioned2022-08-23T16:08:20Z
dc.date.available2022-08-23T16:08:20Z
dc.date.issued2006-03-15
dc.date.submitted2009-09-14
dc.identifier.issn0165-0270 (Print)
dc.identifier.pmid16530271
dc.identifier.urihttp://hdl.handle.net/20.500.14038/32424
dc.description.abstractThe prognosis of vertebrobasilar occlusion is grave and therapeutic options are limited. The aim of the present study was to develop a new model of embolic hindbrain ischemia in the rat that closely resembles the clinical situation and that can be used to study pathophysiology and treatment options. After thoracotomy in 20 male Wistar rats, 15 animals received an injection of in vitro prepared autologous blood clots into the left vertebral artery. Five animals without clot injection served as controls. Neurological deficits were assessed in all animals 2 h after embolism. After 2 h, five animals were sacrificed to measure cerebral blood flow (CBF) by iodo-antipyridine autoradiography, and to calculate early cerebellar swelling by comparison of both hemispheres in brain slices. In these animals, autoradiography revealed ipsilesional brain swelling and significantly reduced blood flow values relative to the contralateral (unaffected) structures. Immunohistology showed the typical pattern of focal cerebral ischemia in the brain stem and/or cerebellum in 7 of 10 animals allowed to recover to 24 h. Hence, successful thromboembolism was achieved in 12 of 15 animals (80%). With this novel model, the pathophysiology and potential treatments of posterior circulation stroke can be investigated.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=16530271&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/j.jneumeth.2006.01.028
dc.rightsCitation: J Neurosci Methods. 2006 Sep 30;156(1-2):1-9. Epub 2006 Mar 10. <a href="http://dx.doi.org/10.1016/j.jneumeth.2006.01.028">Link to article on publisher's site</a>
dc.subjectAnimals
dc.subjectAutoradiography
dc.subjectBrain Edema
dc.subjectCerebral Infarction
dc.subjectCerebrovascular Circulation
dc.subjectDisease Models, Animal
dc.subjectImmunohistochemistry
dc.subjectIntracranial Embolism
dc.subjectMale
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectStroke
dc.subjectThromboembolism
dc.subjectNervous System Diseases
dc.subjectNeurology
dc.titleA new model of thromboembolic stroke in the posterior circulation of the rat
dc.typeJournal Article
dc.source.journaltitleJournal of neuroscience methods
dc.source.volume156
dc.source.issue1-2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_mdphd/7
dc.identifier.contextkey1004106
html.description.abstract<p>The prognosis of vertebrobasilar occlusion is grave and therapeutic options are limited. The aim of the present study was to develop a new model of embolic hindbrain ischemia in the rat that closely resembles the clinical situation and that can be used to study pathophysiology and treatment options. After thoracotomy in 20 male Wistar rats, 15 animals received an injection of in vitro prepared autologous blood clots into the left vertebral artery. Five animals without clot injection served as controls. Neurological deficits were assessed in all animals 2 h after embolism. After 2 h, five animals were sacrificed to measure cerebral blood flow (CBF) by iodo-antipyridine autoradiography, and to calculate early cerebellar swelling by comparison of both hemispheres in brain slices. In these animals, autoradiography revealed ipsilesional brain swelling and significantly reduced blood flow values relative to the contralateral (unaffected) structures. Immunohistology showed the typical pattern of focal cerebral ischemia in the brain stem and/or cerebellum in 7 of 10 animals allowed to recover to 24 h. Hence, successful thromboembolism was achieved in 12 of 15 animals (80%). With this novel model, the pathophysiology and potential treatments of posterior circulation stroke can be investigated.</p>
dc.identifier.submissionpathgsbs_mdphd/7
dc.contributor.departmentGraduate School of Biomedical Sciences, MD/PhD Program
dc.contributor.departmentDepartment of Neurology
dc.source.pages1-9
dc.contributor.studentKenneth Sicard


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