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Multiple copies of the bone-specific osteocalcin gene in mouse and rat

dc.contributor.authorRahman, Shamim
dc.contributor.authorOberdorf, Annette M.
dc.contributor.authorMontecino, Martin A.
dc.contributor.authorTanhauser, Susan M.
dc.contributor.authorLian, Jane B.
dc.contributor.authorStein, Gary S.
dc.contributor.authorLaipis, Philip J.
dc.contributor.authorStein, Janet L.
dc.date2022-08-11T08:08:47.000
dc.date.accessioned2022-08-23T16:08:24Z
dc.date.available2022-08-23T16:08:24Z
dc.date.issued1993-12-01
dc.date.submitted2008-11-26
dc.identifier.citation<p>Endocrinology. 1993 Dec;133(6):3050-3.</p>
dc.identifier.issn0013-7227 (Print)
dc.identifier.doi10.1210/endo.133.6.8243336
dc.identifier.pmid8243336
dc.identifier.urihttp://hdl.handle.net/20.500.14038/32438
dc.description.abstractThe osteocalcin (OC) gene was initially described as a single copy gene encoding the bone specific vitamin K dependent and vitamin D regulated protein. We report here the presence of multiple copies of the gene in mouse and rat. Southern blot analysis and restriction mapping of genomic DNA from several strains of mice indicated the presence of at least three copies of the OC coding sequence within a 19 kb fragment. Two closely linked OC genes contain the proximal promoter region with intact coding sequences. The third potential OC gene includes a 3.5 kb insert between an OC promoter-like region and a coding region that has several amino acid substitutions distributed among functional domains when compared with the normal gene. The 940 nucleotides upstream of the modified coding region lack the well defined 5' regulatory elements that support basal and hormone-responsive transcriptional control. In rats either one or more OC genes were observed in different strains or in Sprague Dawley rats obtained from different suppliers.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8243336&dopt=Abstract">Link to article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1210/endo.133.6.8243336
dc.subjectAmino Acid Sequence; Animals; Base Sequence; Blotting, Southern; Bone and Bones; *Genes; Mice; Mice, Inbred Strains; Molecular Sequence Data; *Multigene Family; Osteocalcin; Rats; Rats, Inbred Strains
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleMultiple copies of the bone-specific osteocalcin gene in mouse and rat
dc.typeJournal Article
dc.source.journaltitleEndocrinology
dc.source.volume133
dc.source.issue6
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1008
dc.identifier.contextkey673225
html.description.abstract<p>The osteocalcin (OC) gene was initially described as a single copy gene encoding the bone specific vitamin K dependent and vitamin D regulated protein. We report here the presence of multiple copies of the gene in mouse and rat. Southern blot analysis and restriction mapping of genomic DNA from several strains of mice indicated the presence of at least three copies of the OC coding sequence within a 19 kb fragment. Two closely linked OC genes contain the proximal promoter region with intact coding sequences. The third potential OC gene includes a 3.5 kb insert between an OC promoter-like region and a coding region that has several amino acid substitutions distributed among functional domains when compared with the normal gene. The 940 nucleotides upstream of the modified coding region lack the well defined 5' regulatory elements that support basal and hormone-responsive transcriptional control. In rats either one or more OC genes were observed in different strains or in Sprague Dawley rats obtained from different suppliers.</p>
dc.identifier.submissionpathgsbs_sp/1008
dc.contributor.departmentDepartment of Cell Biology
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages3050-3


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