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DNA immunization for influenza virus: studies using hemagglutinin- and nucleoprotein-expressing DNAs

dc.contributor.authorRobinson, Harriet L.
dc.contributor.authorBoyle, Christine Margaret
dc.contributor.authorFeltquate, David Marc
dc.contributor.authorMorin, Merribeth J.
dc.contributor.authorSantoro, Joseph C.
dc.contributor.authorWebster, Robert G.
dc.date2022-08-11T08:08:47.000
dc.date.accessioned2022-08-23T16:08:34Z
dc.date.available2022-08-23T16:08:34Z
dc.date.issued1997-08-01
dc.date.submitted2008-12-08
dc.identifier.citation<p>J Infect Dis. 1997 Aug;176 Suppl 1:S50-5.</p>
dc.identifier.issn0022-1899 (Print)
dc.identifier.doi10.1086/514176
dc.identifier.pmid9240695
dc.identifier.urihttp://hdl.handle.net/20.500.14038/32478
dc.description.abstractDNA-based immunizations have been used to analyze the ability of DNA-expressed hemagglutinin (HA) and nucleoprotein (NP) to protect BALB/c mice against a homologous influenza virus, A/PR/8/34 (H1N1), challenge. The HA DNA, but not the NP DNA, protected mice against the lethal viral challenge. For the HA DNA, single gene gun inoculations of 0.04 microg and boosted inoculations of 0.004 microg of DNA raised complete protection. For the NP DNA, boosted gene gun immunizations of 0.4 microg of DNA and boosted intradermal or intramuscular injections of 50 microg of DNA failed to protect. The protection elicited by the HA DNA vaccine correlated with the titers of neutralizing antibody.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=9240695&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1086/514176
dc.subjectAnimals; Antibodies, Viral; Biolistics; Female; Hemagglutinin Glycoproteins, Influenza Virus; Immunization; Influenza Vaccines; Lung; Mice; Mice, Inbred BALB C; Nucleoproteins; Orthomyxoviridae Infections; *RNA-Binding Proteins; Vaccines, DNA; Viral Core Proteins
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleDNA immunization for influenza virus: studies using hemagglutinin- and nucleoprotein-expressing DNAs
dc.typeJournal Article
dc.source.journaltitleThe Journal of infectious diseases
dc.source.volume176 Suppl 1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1047
dc.identifier.contextkey677767
html.description.abstract<p>DNA-based immunizations have been used to analyze the ability of DNA-expressed hemagglutinin (HA) and nucleoprotein (NP) to protect BALB/c mice against a homologous influenza virus, A/PR/8/34 (H1N1), challenge. The HA DNA, but not the NP DNA, protected mice against the lethal viral challenge. For the HA DNA, single gene gun inoculations of 0.04 microg and boosted inoculations of 0.004 microg of DNA raised complete protection. For the NP DNA, boosted gene gun immunizations of 0.4 microg of DNA and boosted intradermal or intramuscular injections of 50 microg of DNA failed to protect. The protection elicited by the HA DNA vaccine correlated with the titers of neutralizing antibody.</p>
dc.identifier.submissionpathgsbs_sp/1047
dc.contributor.departmentDepartment of Pathology
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pagesS50-5


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