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    Isolation of nlz2 and characterization of essential domains in Nlz family proteins

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    Authors
    Runko, Alexander Peter
    Sagerstrom, Charles G.
    UMass Chan Affiliations
    Department of Biochemistry and Molecular Pharmacology
    Graduate School of Biomedical Sciences
    Document Type
    Journal Article
    Publication Date
    2004-01-08
    Keywords
    Base Sequence; Blotting, Western; Cloning, Molecular; DNA Primers; Glutathione Transferase; Molecular Sequence Data; Proteins; Reverse Transcriptase Polymerase Chain Reaction; Structure-Activity Relationship; Zinc Fingers
    Life Sciences
    Medicine and Health Sciences
    
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    Link to Full Text
    http://dx.doi.org/10.1074/jbc.M310076200
    Abstract
    In this study, we first cloned nlz2, a second zebrafish member of the nlz-related zinc-finger gene family. nlz2 was expressed together with nlz1 in a broad posterior domain during gastrula stages as well as at the midbrain-hindbrain boundary and in the hindbrain caudal to rhombomere 4 during segmentation. nlz2 was also expressed in regions distinct from nlz1, notably in the forebrain, midbrain, and trunk. Misexpression of nlz2 in zebrafish embryos disrupted gene expression in the rostral hindbrain, similar to the effect of misexpressing nlz1. We next compared the nlz1 and nlz2 sequences to identify and characterize domains conserved within this family. We found a C-terminal domain required for nuclear localization and two conserved domains (the Sp motif and a putative C(2)H(2) zinc finger) required for nlz1 function. We also demonstrate that Nlz1 self-associated via its C terminus, interacted with Nlz2, and bound to histone deacetylases. Last, we found two forms of Nlz1 generated from alternative translation initiation sites in vivo. These forms have distinct activities, apparently depending on the function of the N-terminal Sp motif. Our data demonstrate that nlz2 functions similarly to nlz1 and define conserved domains essential for nuclear localization, self-association, and corepressor binding in this novel family of zinc-finger genes.
    Source
    J Biol Chem. 2004 Mar 19;279(12):11917-25. Epub 2004 Jan 6. Link to article on publisher's site
    DOI
    10.1074/jbc.M310076200
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/32486
    PubMed ID
    14709556
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1074/jbc.M310076200
    Scopus Count
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