• Login
    View Item 
    •   Home
    • UMass Chan Student Research and Publications
    • Morningside Graduate School of Biomedical Sciences
    • Morningside Graduate School of Biomedical Sciences Scholarly Publications
    • View Item
    •   Home
    • UMass Chan Student Research and Publications
    • Morningside Graduate School of Biomedical Sciences
    • Morningside Graduate School of Biomedical Sciences Scholarly Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of eScholarship@UMassChanCommunitiesPublication DateAuthorsUMass Chan AffiliationsTitlesDocument TypesKeywordsThis CollectionPublication DateAuthorsUMass Chan AffiliationsTitlesDocument TypesKeywords

    My Account

    LoginRegister

    Help

    AboutSubmission GuidelinesData Deposit PolicySearchingAccessibilityTerms of UseWebsite Migration FAQ

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Temporal recruitment of CCAAT/enhancer-binding proteins to early and late adipogenic promoters in vivo

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Salma, Nunciada
    Xiao, Hengyi
    Imbalzano, Anthony N.
    UMass Chan Affiliations
    Department of Cell Biology
    Graduate School of Biomedical Sciences
    Document Type
    Journal Article
    Publication Date
    2006-02-08
    Keywords
    3T3-L1 Cells; Adipose Tissue; Animals; Base Sequence; Blotting, Western; CCAAT-Enhancer-Binding Proteins; Cell Differentiation; DNA Primers; Mice
    Life Sciences
    Medicine and Health Sciences
    
    Metadata
    Show full item record
    Link to Full Text
    http://dx.doi.org/10.1677/jme.1.01918
    Abstract
    The CCAAT/enhancer-binding protein (C/EBP) family of transcriptional regulators is critically important for the activation of adipogenic genes during differentiation. The C/EBPbeta and delta isoforms are rapidly induced upon adipocyte differentiation and are responsible for activating the adipogenic regulators C/EBPalpha and peroxisome proliferator activated receptor (PPAR)gamma2, which together activate the majority of genes expressed in differentiating adipocytes. However, mitosis is required following the induction of adipogenesis, and the activation of C/EBPalpha and PPARgamma2 gene expression is delayed until cell division is underway. Previous studies have used electromobility shift assays to suggest that this delay is due, at least in part, to a delay between the induction of C/EBPbeta protein levels and the acquisition of DNA binding capacity by C/EBPbeta. Here we used in vivo chromatin immunoprecipitation analysis of the C/EBPalpha, PPARgamma2, resistin, adiponectin, and leptin promoters to examine the kinetics of C/EBP protein binding to adipogenic genes in differentiating cells. In contrast to prior studies, we determined that C/EBPbeta and delta were bound to endogenous regulatory sequences controlling the expression of these genes within 1-4 h of adipogenic induction. These results indicated that C/EBPbeta and delta bind not only to genes that are induced early in the adipogenic process but also to genes that are induced much later during differentiation, without a delay between induction of C/EBP protein levels and DNA binding by these proteins. We also showed that each of the genes examined undergoes a transition in vivo from early occupancy by C/EBPbeta and delta to occupancy by C/EBPalpha at times that correlate with the induction of C/EBPalpha protein levels, demonstrating the generality of the transition during adipogenesis and indicating that the binding of specific C/EBP isoforms does not correlate with timing of expression from each gene. We have concluded that C/EBP family members bind to adipogenic genes in vivo in a manner that follows the induction of C/EBP protein synthesis.
    Source
    J Mol Endocrinol. 2006 Feb;36(1):139-51. Link to article on publisher's site
    DOI
    10.1677/jme.1.01918
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/32503
    PubMed ID
    16461934
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1677/jme.1.01918
    Scopus Count
    Collections
    Morningside Graduate School of Biomedical Sciences Scholarly Publications

    entitlement

    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Lamar Soutter Library, UMass Chan Medical School | 55 Lake Avenue North | Worcester, MA 01655 USA
    Quick Guide | escholarship@umassmed.edu
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.