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dc.contributor.authorSalma, Nunciada
dc.contributor.authorXiao, Hengyi
dc.contributor.authorMueller, Elisabetta
dc.contributor.authorImbalzano, Anthony N.
dc.date2022-08-11T08:08:47.000
dc.date.accessioned2022-08-23T16:08:40Z
dc.date.available2022-08-23T16:08:40Z
dc.date.issued2004-05-15
dc.date.submitted2008-12-09
dc.identifier.citationMol Cell Biol. 2004 Jun;24(11):4651-63. <a href="http://dx.doi.org/10.1128/MCB.24.11.4651-4663.2004">Link to article on publisher's site</a>
dc.identifier.issn0270-7306 (Print)
dc.identifier.doi10.1128/MCB.24.11.4651-4663.2004
dc.identifier.pmid15143161
dc.identifier.urihttp://hdl.handle.net/20.500.14038/32504
dc.description.abstractThe peroxisome proliferator-activated receptor gamma (PPARgamma) regulates adipogenesis, lipid metabolism, and glucose homeostasis, and roles have emerged for this receptor in the pathogenesis and treatment of diabetes, atherosclerosis, and cancer. We report here that induction of the PPARgamma activator and adipogenesis forced by overexpression of adipogenic regulatory proteins is blocked upon expression of dominant-negative BRG1 or hBRM, the ATPase subunits of distinct SWI/SNF chromatin-remodeling enzymes. We demonstrate that histone hyperacetylation and the binding of C/EBP activators, polymerase II (Pol II), and general transcription factors (GTFs) initially occurred at the inducible PPARgamma2 promoter in the absence of SWI/SNF function. However, the polymerase and GTFs were subsequently lost from the promoter in cells expressing dominant-negative SWI/SNF, explaining the inhibition of PPARgamma2 expression. To corroborate these data, we analyzed interactions at the PPARgamma2 promoter in differentiating preadipocytes. Changes in promoter structure, histone hyperacetylation, and binding of C/EBP activators, Pol II, and most GTFs preceded the interaction of SWI/SNF enzymes with the PPARgamma2 promoter. However, transcription of the PPARgamma2 gene occurred only upon subsequent association of SWI/SNF and TFIIH with the promoter. Thus, induction of the PPARgamma nuclear hormone receptor during adipogenesis requires SWI/SNF enzymes to facilitate preinitiation complex function.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=15143161&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1128/MCB.24.11.4651-4663.2004
dc.subjectAdipose Tissue; *Cell Differentiation; Chromosomal Proteins, Non-Histone; Enzymes; Fibroblasts; Humans; Promoter Regions (Genetics); Receptors, Cytoplasmic and Nuclear; Transcription Factor TFIIH; Transcription Factors; Transcription Factors, TFII
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleTemporal recruitment of transcription factors and SWI/SNF chromatin-remodeling enzymes during adipogenic induction of the peroxisome proliferator-activated receptor gamma nuclear hormone receptor
dc.typeJournal Article
dc.source.journaltitleMolecular and cellular biology
dc.source.volume24
dc.source.issue11
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1071
dc.identifier.contextkey678838
html.description.abstract<p>The peroxisome proliferator-activated receptor gamma (PPARgamma) regulates adipogenesis, lipid metabolism, and glucose homeostasis, and roles have emerged for this receptor in the pathogenesis and treatment of diabetes, atherosclerosis, and cancer. We report here that induction of the PPARgamma activator and adipogenesis forced by overexpression of adipogenic regulatory proteins is blocked upon expression of dominant-negative BRG1 or hBRM, the ATPase subunits of distinct SWI/SNF chromatin-remodeling enzymes. We demonstrate that histone hyperacetylation and the binding of C/EBP activators, polymerase II (Pol II), and general transcription factors (GTFs) initially occurred at the inducible PPARgamma2 promoter in the absence of SWI/SNF function. However, the polymerase and GTFs were subsequently lost from the promoter in cells expressing dominant-negative SWI/SNF, explaining the inhibition of PPARgamma2 expression. To corroborate these data, we analyzed interactions at the PPARgamma2 promoter in differentiating preadipocytes. Changes in promoter structure, histone hyperacetylation, and binding of C/EBP activators, Pol II, and most GTFs preceded the interaction of SWI/SNF enzymes with the PPARgamma2 promoter. However, transcription of the PPARgamma2 gene occurred only upon subsequent association of SWI/SNF and TFIIH with the promoter. Thus, induction of the PPARgamma nuclear hormone receptor during adipogenesis requires SWI/SNF enzymes to facilitate preinitiation complex function.</p>
dc.identifier.submissionpathgsbs_sp/1071
dc.contributor.departmentDepartment of Cell Biology
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages4651-63


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