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    Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathways

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    Authors
    Schwarz, Dianne S.
    Hutvagner, Gyorgy
    Haley, Benjamin
    Zamore, Phillip D.
    UMass Chan Affiliations
    Department of Biochemistry and Molecular Pharmacology
    Graduate School of Biomedical Sciences
    Document Type
    Journal Article
    Publication Date
    2002-11-01
    Keywords
    Animals; Drosophila melanogaster; Endoribonucleases; *Gene Expression Regulation; Genes, Insect; Hela Cells; Humans; Phosphates; RNA Interference; RNA, Double-Stranded; RNA, Small Interfering; Ribonuclease III
    Life Sciences
    Medicine and Health Sciences
    
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    Link to Full Text
    https://doi.org/10.1016/S1097-2765(02)00651-2
    Abstract
    In Drosophila, two features of small interfering RNA (siRNA) structure--5' phosphates and 3' hydroxyls--are reported to be essential for RNA interference (RNAi). Here, we show that as in Drosophila, a 5' phosphate is required for siRNA function in human HeLa cells. In contrast, we find no evidence in flies or humans for a role in RNAi for the siRNA 3' hydroxyl group. Our in vitro data suggest that in both flies and mammals, each siRNA guides endonucleolytic cleavage of the target RNA at a single site. We conclude that the underlying mechanism of RNAi is conserved between flies and mammals and that RNA-dependent RNA polymerases are not required for RNAi in these organisms.
    Source

    Mol Cell. 2002 Sep;10(3):537-48.

    DOI
    10.1016/S1097-2765(02)00651-2
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/32518
    PubMed ID
    12408822
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    Link to Article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1016/S1097-2765(02)00651-2
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