Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathways
UMass Chan AffiliationsDepartment of Biochemistry and Molecular Pharmacology
Graduate School of Biomedical Sciences
Document TypeJournal Article
KeywordsAnimals; Drosophila melanogaster; Endoribonucleases; *Gene Expression Regulation; Genes, Insect; Hela Cells; Humans; Phosphates; RNA Interference; RNA, Double-Stranded; RNA, Small Interfering; Ribonuclease III
Medicine and Health Sciences
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AbstractIn Drosophila, two features of small interfering RNA (siRNA) structure--5' phosphates and 3' hydroxyls--are reported to be essential for RNA interference (RNAi). Here, we show that as in Drosophila, a 5' phosphate is required for siRNA function in human HeLa cells. In contrast, we find no evidence in flies or humans for a role in RNAi for the siRNA 3' hydroxyl group. Our in vitro data suggest that in both flies and mammals, each siRNA guides endonucleolytic cleavage of the target RNA at a single site. We conclude that the underlying mechanism of RNAi is conserved between flies and mammals and that RNA-dependent RNA polymerases are not required for RNAi in these organisms.
Mol Cell. 2002 Sep;10(3):537-48.
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/32518