Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathways
| dc.contributor.author | Schwarz, Dianne S. | |
| dc.contributor.author | Hutvagner, Gyorgy | |
| dc.contributor.author | Haley, Benjamin | |
| dc.contributor.author | Zamore, Phillip D. | |
| dc.date | 2022-08-11T08:08:48.000 | |
| dc.date.accessioned | 2022-08-23T16:08:43Z | |
| dc.date.available | 2022-08-23T16:08:43Z | |
| dc.date.issued | 2002-11-01 | |
| dc.date.submitted | 2008-12-10 | |
| dc.identifier.citation | <p>Mol Cell. 2002 Sep;10(3):537-48.</p> | |
| dc.identifier.issn | 1097-2765 (Print) | |
| dc.identifier.doi | 10.1016/S1097-2765(02)00651-2 | |
| dc.identifier.pmid | 12408822 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14038/32518 | |
| dc.description.abstract | In Drosophila, two features of small interfering RNA (siRNA) structure--5' phosphates and 3' hydroxyls--are reported to be essential for RNA interference (RNAi). Here, we show that as in Drosophila, a 5' phosphate is required for siRNA function in human HeLa cells. In contrast, we find no evidence in flies or humans for a role in RNAi for the siRNA 3' hydroxyl group. Our in vitro data suggest that in both flies and mammals, each siRNA guides endonucleolytic cleavage of the target RNA at a single site. We conclude that the underlying mechanism of RNAi is conserved between flies and mammals and that RNA-dependent RNA polymerases are not required for RNAi in these organisms. | |
| dc.language.iso | en_US | |
| dc.relation | <p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=12408822&dopt=Abstract">Link to Article in PubMed</a></p> | |
| dc.relation.url | https://doi.org/10.1016/S1097-2765(02)00651-2 | |
| dc.subject | Animals; Drosophila melanogaster; Endoribonucleases; *Gene Expression Regulation; Genes, Insect; Hela Cells; Humans; Phosphates; RNA Interference; RNA, Double-Stranded; RNA, Small Interfering; Ribonuclease III | |
| dc.subject | Life Sciences | |
| dc.subject | Medicine and Health Sciences | |
| dc.title | Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathways | |
| dc.type | Journal Article | |
| dc.source.journaltitle | Molecular cell | |
| dc.source.volume | 10 | |
| dc.source.issue | 3 | |
| dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/gsbs_sp/1085 | |
| dc.identifier.contextkey | 679623 | |
| html.description.abstract | <p>In Drosophila, two features of small interfering RNA (siRNA) structure--5' phosphates and 3' hydroxyls--are reported to be essential for RNA interference (RNAi). Here, we show that as in Drosophila, a 5' phosphate is required for siRNA function in human HeLa cells. In contrast, we find no evidence in flies or humans for a role in RNAi for the siRNA 3' hydroxyl group. Our in vitro data suggest that in both flies and mammals, each siRNA guides endonucleolytic cleavage of the target RNA at a single site. We conclude that the underlying mechanism of RNAi is conserved between flies and mammals and that RNA-dependent RNA polymerases are not required for RNAi in these organisms.</p> | |
| dc.identifier.submissionpath | gsbs_sp/1085 | |
| dc.contributor.department | Department of Biochemistry and Molecular Pharmacology | |
| dc.contributor.department | Graduate School of Biomedical Sciences | |
| dc.source.pages | 537-48 |
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