Cell cycle regulation of the c-Myc transcriptional activation domain
UMass Chan Affiliations
Program in Molecular MedicineDepartment of Biochemistry and Molecular Biology
Graduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
1993-07-01Keywords
Animals; Base Sequence; Binding Sites; CHO Cells; Cell Cycle; Cell Line; Cricetinae; DNA; Gene Expression Regulation; *Genes, myc; Kinetics; Molecular Sequence Data; Phosphorylation; Serine; Trans-Activation (Genetics); *Transcription, GeneticLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
The product of the c-myc gene (c-Myc) is a sequence-specific DNA-binding protein that has previously been demonstrated to be required for cell cycle progression. Here we report that the c-Myc DNA binding site confers cell cycle regulation to a reporter gene in Chinese hamster ovary cells. The observed transactivation was biphasic with a small increase in G1 and a marked increase during the S-to-G2/M transition of the cell cycle. This cell cycle regulation of transactivation potential is accounted for, in part, by regulatory phosphorylation of the c-Myc transactivation domain. Together, these data demonstrate that c-Myc may have an important role in the progression of cells through both the G1 and G2 phases of the cell cycle.Source
Mol Cell Biol. 1993 Jul;13(7):4125-36.
DOI
10.1128/MCB.13.7.4125Permanent Link to this Item
http://hdl.handle.net/20.500.14038/32532PubMed ID
8321217Related Resources
ae974a485f413a2113503eed53cd6c53
10.1128/MCB.13.7.4125