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dc.contributor.authorSigova, Alla A.
dc.contributor.authorRhind, Nicholas R.
dc.contributor.authorZamore, Phillip D.
dc.date2022-08-11T08:08:48.000
dc.date.accessioned2022-08-23T16:08:52Z
dc.date.available2022-08-23T16:08:52Z
dc.date.issued2004-09-17
dc.date.submitted2008-12-11
dc.identifier.citation<p>Genes Dev. 2004 Oct 1;18(19):2359-67. Epub 2004 Sep 15. <a href="http://dx.doi.org/10.1101/gad.1218004">Link to article on publisher's site</a></p>
dc.identifier.issn0890-9369 (Print)
dc.identifier.doi10.1101/gad.1218004
dc.identifier.pmid15371329
dc.identifier.urihttp://hdl.handle.net/20.500.14038/32554
dc.description.abstractThe Schizosaccharomyces pombe genome encodes only one of each of the three major classes of proteins implicated in RNA silencing: Dicer (Dcr1), RNA-dependent RNA polymerase (RdRP; Rdp1), and Argonaute (Ago1). These three proteins are required for silencing at centromeres and for the initiation of transcriptionally silent heterochromatin at the mating-type locus. Here, we show that the introduction of a double-stranded RNA (dsRNA) hairpin corresponding to a green fluorescent protein (GFP) transgene triggers classical RNA interference (RNAi) in S. pombe. That is, GFP silencing triggered by dsRNA reflects a change in the steady-state concentration of GFP mRNA, but not in the rate of GFP transcription. RNAi in S. pombe requires dcr1, rdp1, and ago1, but does not require chp1, tas3, or swi6, genes required for transcriptional silencing. Thus, the RNAi machinery in S. pombe can direct both transcriptional and posttranscriptional silencing using a single Dicer, RdRP, and Argonaute protein. Our findings suggest that these three proteins fulfill a common biochemical function in distinct siRNA-directed silencing pathways.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=15371329&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC522986/
dc.subjectBase Sequence; DNA Primers; Flow Cytometry; Gene Silencing; Green Fluorescent Proteins; Luminescent Proteins; Schizosaccharomyces; Schizosaccharomyces pombe Proteins; Transcription, Genetic
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleA single Argonaute protein mediates both transcriptional and posttranscriptional silencing in Schizosaccharomyces pombe
dc.typeJournal Article
dc.source.journaltitleGenes and development
dc.source.volume18
dc.source.issue19
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1118
dc.identifier.contextkey680291
html.description.abstract<p>The Schizosaccharomyces pombe genome encodes only one of each of the three major classes of proteins implicated in RNA silencing: Dicer (Dcr1), RNA-dependent RNA polymerase (RdRP; Rdp1), and Argonaute (Ago1). These three proteins are required for silencing at centromeres and for the initiation of transcriptionally silent heterochromatin at the mating-type locus. Here, we show that the introduction of a double-stranded RNA (dsRNA) hairpin corresponding to a green fluorescent protein (GFP) transgene triggers classical RNA interference (RNAi) in S. pombe. That is, GFP silencing triggered by dsRNA reflects a change in the steady-state concentration of GFP mRNA, but not in the rate of GFP transcription. RNAi in S. pombe requires dcr1, rdp1, and ago1, but does not require chp1, tas3, or swi6, genes required for transcriptional silencing. Thus, the RNAi machinery in S. pombe can direct both transcriptional and posttranscriptional silencing using a single Dicer, RdRP, and Argonaute protein. Our findings suggest that these three proteins fulfill a common biochemical function in distinct siRNA-directed silencing pathways.</p>
dc.identifier.submissionpathgsbs_sp/1118
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.source.pages2359-67


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